Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China.
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
PLoS One. 2020 Dec 10;15(12):e0243748. doi: 10.1371/journal.pone.0243748. eCollection 2020.
The pre-aerobic process of coking wastewater treatment has strong capacity of decarbonization and detoxification, which contribute to the subsequent dinitrogen of non-carbon source/heterotrophic denitrification. The COD removal rate can reach > 90% in the first aerobic bioreactor of the novel O/H/O coking wastewater treatment system during long-term operation. The physico-chemical characteristics of influent and effluent coking wastewater in the first aerobic bioreactor were analyzed to examine how they correlated with bacterial communities. The diversity of the activated sludge microbial community was investigated using a culture-independent molecular approach. The microbial community functional profiling and detailed pathways were predicted from the 16S rRNA gene-sequencing data by the PICRUSt software and the KEGG database. High-throughput MiSeq sequencing results revealed a distinct microbial composition in the activated sludge of the first aerobic bioreactor of the O/H/O system. Proteobacteria, Bacteroidetes, and Chlorobi were the decarbonization and detoxification dominant phyla with the relative abundance of 84.07 ± 5.45, 10.89 ± 6.31, and 2.96 ± 1.12%, respectively. Thiobacillus, Rhodoplanes, Lysobacter, and Leucobacter were the potential major genera involved in the crucial functional pathways related to the degradation of phenols, cyanide, benzoate, and naphthalene. These results indicated that the comprehensive understanding of the structure and function diversity of the microbial community in the bioreactor will be conducive to the optimal coking wastewater treatment.
焦化废水处理的前置好氧工艺具有较强的脱碳和解毒能力，有助于后续非碳源/异养反硝化的双氮去除。在新型 O/H/O 焦化废水处理系统的第一个好氧生物反应器中，经过长期运行，COD 去除率可达到>90%。分析了第一个好氧生物反应器中进水和出水焦化废水的理化特性，以考察它们与细菌群落的相关性。采用非培养分子方法研究了活性污泥微生物群落的多样性。通过 PICRUSt 软件和 KEGG 数据库，从 16S rRNA 基因测序数据预测微生物群落功能谱和详细途径。高通量 MiSeq 测序结果显示，O/H/O 系统第一个好氧生物反应器中的活性污泥微生物组成明显不同。脱碳和解毒优势菌门为变形菌门、拟杆菌门和绿弯菌门，相对丰度分别为 84.07±5.45%、10.89±6.31%和 2.96±1.12%。硫杆菌属、红平菌属、赖氨酸杆菌属和白色杆菌属是与酚类、氰化物、苯甲酸和萘降解相关的关键功能途径中潜在的主要属。这些结果表明，对生物反应器中微生物群落结构和功能多样性的综合理解将有助于优化焦化废水处理。
