Department of Medical Biochemistry, Medical University of Białystok, Białystok, Poland,
Department of Urology, Medical University of Białystok, Białystok, Poland,
Pathobiology. 2019;86(4):201-207. doi: 10.1159/000499499. Epub 2019 May 24.
Collagenases are enzymes starting collagen degradation. The role of collagenases in renal carcinoma development is not well understood.
Evaluation of collagen content and collagenase expression and activity in human kidney cancers.
Collagen content was measured by the hydroxyproline assay. The expression and the content of collagenases were evaluated by Western blotting and ELISA. Fluorogenic substrate was used to measure enzyme activity.
Collagen content significantly decreases with the progression of kidney cancer. Both collagenases are first present in high molecular complexes in both control and cancer tissue. The healthy part of the kidney contains similar amounts of both collagenases. Collagenase content decreased significantly in tumor tissue with increasing cancer stage. MMP-13 activity is much higher than that of MMP-1 in all tissues investigated. We observed increasing collagenase activity (MMP-1 and MMP-13) with increasing renal cancer grade.
The lower content and higher activity of the collagenases investigated in cancer tissue indicate that most of these enzymes are in active form in renal carcinoma. The lower collagen content in cancer tissue can be explained at least in part by increased collagenase activity.
胶原酶是启动胶原蛋白降解的酶。胶原酶在肾癌发展中的作用尚不清楚。
评估人类肾癌中的胶原含量和胶原酶表达及活性。
通过羟脯氨酸测定法测量胶原含量。通过 Western blot 和 ELISA 评估胶原酶的表达和含量。使用荧光底物测定酶活性。
胶原含量随肾癌的进展显著降低。两种胶原酶最初都以高分子复合物的形式存在于对照和癌组织中。健康的肾脏组织含有相似量的两种胶原酶。随着癌症阶段的增加，肿瘤组织中胶原酶的含量显著降低。MMP-13 的活性在所有研究的组织中均明显高于 MMP-1。我们观察到随着肾细胞癌分级的增加，胶原酶活性（MMP-1 和 MMP-13）增加。
在癌组织中检测到的胶原酶含量较低且活性较高，表明这些酶中的大多数在肾细胞癌中处于活跃状态。癌组织中胶原含量较低至少部分可以解释为胶原酶活性增加。
