Kostiukova N N, Karas' S R
Zh Mikrobiol Epidemiol Immunobiol. 1987 May(5):13-6.
The conditions for the direct hemagglutination test performed to determine the degree of adhesion of C. diphtheriae were defined. For this test sheep red blood cells, trypsin-treated ex tempore, were used. Only newly isolated cultures, subcultured for not more than 2-5 times and stored for not more than 2-7 days or freeze-dried, were employed. The culture to be tested was grown in nutrient agar with 10% of normal horse serum. The test was made in microtitrator round-bottom wells. The mixture of different dilutions of the culture was incubated for 2 hours at 37 degrees C, then left overnight at 4 degrees C. All 147 newly isolated or freeze-dried C. diphtheriae strains under test had different degrees of adhesion. Their adhesive activity was unrelated to their biovar. Toxigenic strains were significantly more active in hemagglutination (53.5 +/- 3.0%) than nontoxigenic ones (23.5 +/- 3.9%). The strains isolated from the nose, irrespective of their biological properties, were more active than those isolated from the pharynx.
确定白喉棒状杆菌黏附程度的直接血凝试验条件已明确。为此试验，使用临时用胰蛋白酶处理过的绵羊红细胞。仅采用新分离的培养物，传代不超过2至5次且保存不超过2至7天或冻干的培养物。待测培养物在含10%正常马血清的营养琼脂中生长。试验在微量滴定板圆底孔中进行。不同稀释度培养物的混合物在37℃孵育2小时，然后在4℃放置过夜。所有147株新分离或冻干的待测白喉棒状杆菌菌株黏附程度各异。它们的黏附活性与其生物变种无关。产毒株的血凝活性（53.5±3.0%）显著高于非产毒株（23.5±3.9%）。从鼻腔分离的菌株，无论其生物学特性如何，比从咽部分离的菌株活性更高。
