Department of Genetics, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Nanomedicine Group, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Curr Mol Med. 2019;19(7):461-472. doi: 10.2174/1566524019666190527083947.
The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced. The best alternatives were the isothermal amplification technologies that those did not require a thermal cycler. In this review, we aimed to describe the most known isothermal amplification techniques for SNP genotyping.
对具有可接受的精度、准确性和成本效益的基因分型技术的需求，以及高通量格式的需求，成为推动新技术不断发展的动力。已经引入了广泛的基于聚合酶链反应 (PCR) 的突变检测技术。最好的选择是不需要热循环仪的等温扩增技术。在这篇综述中，我们旨在描述用于 SNP 基因分型的最知名的等温扩增技术。
