两种针对外周血单核细胞分化抗原的小鼠单克隆抗体可区分M5型急性非淋巴细胞白血病（ANLL）细胞。

Two murine monoclonal antibodies to peripheral blood monocyte differentiation antigens discriminate within M5 acute non-lymphoid leukemia (ANLL) cells.

作者信息

Lopez M, De Rossi G, Santoro L, Mandelli F, Alescio T, Annino L, Pasqualetti D, Siccardi A G, Mottolese M, Natali P G

出版信息

Am J Hematol. 1987 Aug;25(4):361-9. doi: 10.1002/ajh.2830250402.

Abstract

Two murine monoclonal antibodies (MoAbs), LAM3 and LAM7 of the IgG1 isotype, which were produced by immunization with normal peripheral blood monocytes (PBM), were assayed in their specificity by indirect immunofluorescence against a panel of normal as well as leukemic cells. Both LAM3 and LAM7 were reactive with PBM while LAM3 also recognized platelets. Neither MoAb showed reactivity with erythrocytes, granulocytes, or resting and mitogen activated B and T lymphocytes. The reactivity with bone marrow cells correlated with the degree of monocyte contamination. Among the 62 cases of leukemia tested, which included three cases of B-CLL, 19 cases of ALL, and 40 cases of ANLL, both MoAbs reacted highly homogenously only with M5b ANLL cells. These findings indicate that the two MoAbs, which recognize two distinct epitopes, represent useful markers in the differential diagnosis of M5b ANLL.

摘要

通过用正常外周血单核细胞（PBM）免疫制备了两种IgG1同种型的小鼠单克隆抗体（MoAb）LAM3和LAM7，通过间接免疫荧光法针对一组正常细胞以及白血病细胞检测了它们的特异性。LAM3和LAM7均与PBM反应，而LAM3还识别血小板。两种单克隆抗体均未显示与红细胞、粒细胞或静息及有丝分裂原激活的B和T淋巴细胞发生反应。与骨髓细胞的反应性与单核细胞污染程度相关。在所检测的62例白血病病例中，包括3例B - CLL、19例ALL和40例ANLL，两种单克隆抗体仅与M5b型ANLL细胞高度均匀地反应。这些发现表明，这两种识别两个不同表位的单克隆抗体是M5b型ANLL鉴别诊断中的有用标志物。