Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, United States.
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, United States.
Curr Opin Struct Biol. 2019 Aug;57:164-175. doi: 10.1016/j.sbi.2019.03.028. Epub 2019 May 25.
Many biological processes are naturally regulated with spatiotemporal control. In order to perturb and investigate them, optochemical tools have been developed that convey similar spatiotemporal precision. Pivotal to optochemical probes are photolabile protecting groups, so called caging groups, and recent developments have enabled new applications to cellular processes, including cell signaling. This review focuses on the advances made in the field of caging groups and their application in cell signaling through caged molecules such as neurotransmitters, lipids, secondary messengers, and proteins.
许多生物过程都受到时空控制的自然调节。为了干扰和研究这些过程，人们开发了光化学工具，这些工具具有类似的时空精度。光化学探针的关键是光不稳定保护基团，即所谓的笼蔽基团，最近的发展使它们能够应用于细胞过程，包括细胞信号转导。本文综述了笼蔽基团领域的进展及其在通过笼蔽分子（如神经递质、脂质、第二信使和蛋白质）进行细胞信号转导中的应用。
