Knapp Fabienne, Hogenkamp Fabian, Paik Seung-Hyun, Jaeger Karl-Erich, Pietruszka Jörg, Drepper Thomas
Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich GmbH, Jülich, Germany.
Institute of Bioorganic Chemistry, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich GmbH, Jülich, Germany.
Methods Mol Biol. 2025;2840:133-148. doi: 10.1007/978-1-0716-4047-0_10.
Photocaged compounds are chemical conjugates that are designed to release an active molecule upon exposure to light of a specific wavelength. In recent years, photocaged inducer molecules such as caged isopropyl β-D-1-thiogalactopyranoside (cIPTG) have been increasingly used as a powerful tool for light-driven gene expression in bacteria, allowing researchers to precisely and noninvasively tune the expression of specific target genes. In this chapter, we present a guideline for the synthesis of 6-nitropiperonyl photocaged IPTG (NP-cIPTG) as well as its in vivo application as an optochemical on-switch of gene transcription in Escherichia coli and other bacteria.
光笼化合物是一种化学共轭物，其设计目的是在暴露于特定波长的光时释放活性分子。近年来，诸如光笼化异丙基β-D-1-硫代半乳糖苷（cIPTG）等光笼诱导分子已越来越多地用作细菌中光驱动基因表达的强大工具，使研究人员能够精确且无创地调节特定靶基因的表达。在本章中，我们介绍了6-硝基胡椒基光笼化IPTG（NP-cIPTG）的合成指南及其作为大肠杆菌和其他细菌中基因转录的光化学开关在体内的应用。
