背脂脂肪酸组成与候选基因 SNPs 的关联研究突出了 FASN 多态性在大白猪中的作用。

Association study between backfat fatty acid composition and SNPs in candidate genes highlights the effect of FASN polymorphism in large white pigs.

机构信息

Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy.

Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy..

出版信息

Meat Sci. 2019 Oct;156:75-84. doi: 10.1016/j.meatsci.2019.05.013. Epub 2019 May 14.

DOI:10.1016/j.meatsci.2019.05.013

PMID:31132591

Abstract

The objective of the study was to test the associations between backfat fatty acid composition (FAC) in a sample of 536 Italian Large White pigs and single nucleotide polymorphisms (SNPs) located in candidate genes, using univariate and multivariate approaches. The strongest associations were identified for the SNP AY183428 c.265T>C in Fatty acid synthase (FASN) gene, with the T allele linked to lower backfat contents of stearic (P = 0.003) and arachidic (P < 0.0001) acids, and increased amounts of dihomo-γ-linolenic (P = 0.003) and arachidonic (P = 0.009) acids. These associations were in agreement with the results of a multivariate analysis performed on backfat FAC, and an in silico analysis of the sequence flanking FASN SNP suggested that the T allele may disrupt a putative exonic splicing enhancer sequence therefore possibly affecting FASN activity. If the results will be further confirmed, the studied FASN SNP could be of particular interest for better understanding the regulative patterns controlling porcine backfat FAC.

摘要

本研究的目的是测试 536 头意大利大白猪样本中背脂脂肪酸组成（FAC）与候选基因中单个核苷酸多态性（SNP）之间的关联，采用单变量和多变量方法。在脂肪酸合酶（FASN）基因中，AY183428 c.265T>C SNP 与硬脂酸（P=0.003）和花生酸（P<0.0001）含量较低的背脂以及二高-γ-亚麻酸（P=0.003）和花生四烯酸（P=0.009）含量增加的等位基因 T 之间存在最强的关联。这些关联与背脂 FAC 的多变量分析结果一致，并且对 FASN SNP 侧翼序列的计算机分析表明，T 等位基因可能破坏了一个假定的外显子剪接增强子序列，因此可能影响 FASN 活性。如果这些结果得到进一步证实，所研究的 FASN SNP 可能特别有助于更好地了解控制猪背脂 FAC 的调节模式。