细胞学和转录组分析表明，Sb 花粉不育位点的相互作用导致与同源四倍体水稻杂种中高花粉不育相关的重要减数分裂相关基因下调。
Cytological and transcriptome analysis reveal that interaction at Sb pollen sterility locus cause down-regulation of important meiosis-related genes associated with high pollen sterility in autotetraploid rice hybrids.
机构信息
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China; College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China; College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
出版信息
Plant Physiol Biochem. 2019 Aug;141:73-82. doi: 10.1016/j.plaphy.2019.05.019. Epub 2019 May 21.
Polyploidy could increase the interactions of pollen sterility loci and Sb locus interaction cause higher pollen abortion than other loci. Therefore, we focused on the interaction at Sb pollen sterility locus in autotetraploid rice compared to diploid rice hybrid using the near-isogenic lines in the present study. Cytological observations indicated that interaction at Sb locus cause high pollen sterility (69.9%) and abnormal chromosome behavior (37.02%) at Metaphase II in autotetraploid rice hybrid. A total of 139 meiosis-related or meiosis stage-specific genes were detected in the autotetraploid rice hybrid harboring interaction at Sb locus and 27 of these meiosis-related or specific genes displayed significant down-regulation, including four pollen fertility related genes (Rad51, XRI1, PSS1 and MIL1). These results revealed a stronger interaction at Sb pollen sterility locus than other loci, which cause down-regulation of many important meiosis-related genes that were associated with higher pollen sterility in autotetraploid rice hybrids.
多倍体可以增加花粉不育基因座之间的相互作用，而 Sb 基因座的相互作用导致花粉败育率高于其他基因座。因此，我们在本研究中使用近等基因系，重点研究了四倍体水稻杂种中与 Sb 花粉不育基因座的相互作用，与二倍体水稻杂种相比。细胞学观察表明，Sb 基因座的相互作用导致四倍体水稻杂种中期花粉不育率（69.9%）和染色体异常行为（37.02%）较高。在含有 Sb 基因座相互作用的四倍体水稻杂种中检测到 139 个与减数分裂相关或减数分裂阶段特异性基因，其中 27 个与减数分裂相关或特异性基因显示出显著下调，包括四个花粉育性相关基因（Rad51、XRI1、PSS1 和 MIL1）。这些结果表明，Sb 花粉不育基因座的相互作用强于其他基因座，导致许多与四倍体水稻杂种中花粉不育相关的重要减数分裂相关基因下调。
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