Kamara Nabieu, Jiao Yamin, Huang Weicong, Cao Lichong, Zhu Lianjun, Zhao Chongchong, Huang Xu, Shivute Fimanekeni Ndaitavela, Liu Xiangdong, Wu Jinwen, Shahid Muhammad Qasim
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Front Plant Sci. 2023 Jul 17;14:1229870. doi: 10.3389/fpls.2023.1229870. eCollection 2023.
We aimed to investigate the genetic defects related to pollen development and infertility in , a novel tetraploid rice germplasm known as Neo-tetraploid rice. This rice variety was created through the crossbreeding and selective breeding of various autotetraploid rice lines and has previously shown high fertility. Our previous research has revealed that the gene, encoding a eukaryotic translation initiation factor 3 subunit E, regulates pollen fertility. However, the underlying mechanism behind this fertility is yet to be understood. To shed light on this matter, we performed a combined cytological and transcriptome analysis of the gene. Cytological analysis indicated that underwent abnormal tapetal cells, microspore, and middle layer development, which led to pollen abortion and ultimately to male sterility. Genetic analysis revealed that the F plants showed normal fertility and an obvious advantage for seed setting compared to . Global gene expression analysis in revealed a total of 7545 genes were detected at the meiosis stage, and 3925 and 3620 displayed upregulation and downregulation, respectively. The genes were significantly enriched for the gene ontology (GO) term "carbohydrate metabolic process. Moreover, 9 genes related to tapetum or pollen fertility showed down-regulation, such as (ATP Binding Cassette G26), (Thermosensitive Male Sterility), (Programmed cell death regulatory), (Kinesin Motor), (Metallothionein), and (Atypical strictosidine synthase), which were validated by qRT-PCR. Further analyses of DEGs identified nine down-regulated transcription factor genes related to pollen development. is an important regulator of the development of tapetum and microspore. The regulatory gene network described in this study may offer important understandings into the molecular processes that underlie fertility control in tetraploid rice.
我们旨在研究与一种名为新四倍体水稻的新型四倍体水稻种质花粉发育和不育相关的基因缺陷。该水稻品种是通过各种同源四倍体水稻品系的杂交育种和选择育种培育而成的，此前已表现出高育性。我们之前的研究表明，编码真核翻译起始因子3亚基E的基因调节花粉育性。然而，这种育性背后的潜在机制尚不清楚。为了阐明这一问题，我们对该基因进行了细胞学和转录组联合分析。细胞学分析表明，该基因经历了绒毡层细胞、小孢子和中层发育异常，导致花粉败育并最终导致雄性不育。遗传分析表明，与相比，F植株表现出正常育性且结实率具有明显优势。对该基因的全基因组表达分析显示，在减数分裂阶段共检测到7545个基因，其中3925个和3620个分别上调和下调。这些基因在基因本体（GO）术语“碳水化合物代谢过程”中显著富集。此外，9个与绒毡层或花粉育性相关的基因显示下调，如（ATP结合盒G26）、（热敏雄性不育）、（程序性细胞死亡调节）、（驱动蛋白）、（金属硫蛋白）和（非典型 strictosidine 合酶），这些基因通过qRT-PCR得到验证。对差异表达基因的进一步分析确定了9个与花粉发育相关的下调转录因子基因。该基因是绒毡层和小孢子发育的重要调节因子。本研究中描述的调控基因网络可能为四倍体水稻育性控制的分子过程提供重要见解。
