Zalipour Mehrdad, Esfahani Bahram Nasr, Havaei Seyed Asghar
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Hezar Jarib St, Isfahan, Iran.
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
BMC Res Notes. 2019 May 27;12(1):292. doi: 10.1186/s13104-019-4339-4.
Enterococcus faecalis as part of the normal floras of human gastrointestinal and genitourinary tracts are an important cause of nosocomial infections. The present study aimed to investigate the prevalence of genes encoding antimicrobial resistance and genetic relatedness of clinical isolates of E. faecalis among Iranian hospitalized patients.
Antibiotic susceptibility testing results indicated that 53 (22.8%) out of 232 E. faecalis isolates were vancomycin resistant (MIC ≥ 256 μg/ml). All of the 53 vancomycin-resistant E. faecalis isolates carried the vanA and ermB genes; whereas aac (6')-Ie aph (2″), msrA, and ermA gene were found in 96.2%, 30.2% and 3.8% of vancomycin-resistant isolates, respectively. ERIC-PCR typing revealed that 53 vancomycin-resistant isolates were classified into 14 ERIC types. In our results, the high level of resistance to gentamicin, erythromycin and vancomycin in enterococci isolates were mainly related to the presence of aac (6')-Ie aph (2″), ermB and vanA genes, respectively. Meanwhile, ERIC-PCR analysis demonstrated that most of the evaluated isolates have a close genetic relatedness.
粪肠球菌作为人类胃肠道和泌尿生殖道正常菌群的一部分，是医院感染的重要原因。本研究旨在调查伊朗住院患者中粪肠球菌临床分离株的抗菌耐药基因流行情况及遗传相关性。
药敏试验结果表明，232株粪肠球菌分离株中有53株（22.8%）对万古霉素耐药（MIC≥256μg/ml）。所有53株万古霉素耐药粪肠球菌分离株均携带vanA和ermB基因；而在96.2%、30.2%和3.8%的万古霉素耐药分离株中分别发现了aac(6')-Ie aph(2″)、msrA和ermA基因。ERIC-PCR分型显示53株万古霉素耐药分离株被分为14种ERIC型。在我们的结果中，肠球菌分离株对庆大霉素、红霉素和万古霉素的高耐药水平分别主要与aac(6')-Ie aph(2″)、ermB和vanA基因的存在有关。同时，ERIC-PCR分析表明，大多数评估的分离株具有密切的遗传相关性。
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