Dolen J G, Park B H
Immunol Commun. 1978;7(6):677-80. doi: 10.3109/08820137809068728.
A micro-method for enumerating B-lymphocytes in human peripheral blood is described, using formation of rosettes with mouse red blood cells as a B-cell marker. The optimal conditions for the enumeration of mouse red cell rosette forming cells have been defined. By this method, it was possible to utilize less than 1ml of peripheral blood (venous or capillary) for simultaneous enumeration of both T-cell and B-cell rosettes. Our study of human lymphoid cell lines, thymic cells, lymphocytes from agammaglobulinemia, and severe combined immunodeficiency has provided additional evidence that lymphocyte rosetting with mouse red blood cells represent B-lymphocytes.
本文描述了一种用于计数人外周血中B淋巴细胞的微量方法，该方法利用与小鼠红细胞形成花环作为B细胞标志物。已确定了计数小鼠红细胞花环形成细胞的最佳条件。通过这种方法，使用少于1ml的外周血（静脉血或毛细血管血）就可以同时计数T细胞和B细胞花环。我们对人淋巴细胞系、胸腺细胞、无丙种球蛋白血症患者的淋巴细胞以及严重联合免疫缺陷患者的淋巴细胞的研究提供了更多证据，表明与小鼠红细胞形成花环的淋巴细胞代表B淋巴细胞。
