Eremin O, Binns R M
Immunology. 1982 Oct;47(2):321-7.
Inclusion of 14% Ficoll in the assay of rosette formation of human lymphocytes with mouse red blood cells (RBC) markedly enhances the cellular interaction. Both the strength of the rosettes (number of attached RBC) and the number of reacting lymphocytes are increased significantly, irrespective of the lymphocyte source--blood, lymph node and tonsil. In the presence of Ficoll, most of the sIg-bearing B lymphocytes [expressing receptors for Fc(IgG) and/or C3] and a significant number of the sheep RBC-rosetting T lymphocytes (particularly the nylon wool non-adherent subset from the tonsil), express receptors for the mouse erythrocyte.
在用人淋巴细胞与小鼠红细胞(RBC)进行玫瑰花结形成试验中加入14%的聚蔗糖,可显著增强细胞间相互作用。无论淋巴细胞来源是血液、淋巴结还是扁桃体,玫瑰花结的强度(附着的红细胞数量)和反应性淋巴细胞数量均显著增加。在聚蔗糖存在的情况下,大多数带有表面免疫球蛋白(sIg)的B淋巴细胞[表达Fc(IgG)和/或C3受体]以及大量与绵羊红细胞形成玫瑰花结的T淋巴细胞(特别是来自扁桃体的尼龙毛非黏附亚群),均表达小鼠红细胞受体。