Raorane Chaitany Jayprakash, Lee Jin-Hyung, Kim Yong-Guy, Rajasekharan Satish Kumar, García-Contreras Rodolfo, Lee Jintae
School of Chemical Engineering, Yeungnam University, Gyeongsan, South Korea.
Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico.
Front Microbiol. 2019 May 8;10:990. doi: 10.3389/fmicb.2019.00990. eCollection 2019.
is well adapted to hospital environments, and the persistence of its chronic infections is mainly due to its ability to form biofilms resistant to conventional antibiotics and host immune systems. Hence, the inhibitions of biofilm formation and virulence characteristics provide other means of addressing infections. In this study, the antibiofilm activities of twelve flavonoids were initially investigated. Three most active flavonoids, namely, fisetin, phloretin, and curcumin, dose-dependently inhibited biofilm formation by a reference strain and by several clinical isolates, including four multidrug-resistant isolates. Furthermore, the antibiofilm activity of curcumin (the most active flavonoid) was greater than that of the well-known biofilm inhibitor gallium nitrate. Curcumin inhibited pellicle formation and the surface motility of . Interestingly, curcumin also showed antibiofilm activity against and mixed cultures of and . molecular docking of the biofilm response regulator BfmR showed that the binding efficacy of flavonoids with BfmR was correlated with antibiofilm efficacy. In addition, curcumin treatment diminished virulence in an model without cytotoxicity. The study shows curcumin and other flavonoids have potential for controlling biofilm formation by and the virulence of .
它非常适应医院环境，其慢性感染的持续存在主要归因于它形成对传统抗生素和宿主免疫系统具有抗性的生物膜的能力。因此，抑制生物膜形成和毒力特性提供了应对感染的其他方法。在本研究中，最初研究了十二种类黄酮的抗生物膜活性。三种活性最高的类黄酮，即非瑟酮、根皮素和姜黄素，对一株参考菌株和包括四种多重耐药菌株在内的几种临床分离株的生物膜形成具有剂量依赖性抑制作用。此外，姜黄素（活性最高的类黄酮）的抗生物膜活性大于著名的生物膜抑制剂硝酸镓。姜黄素抑制菌膜形成和[具体细菌名称]的表面运动性。有趣的是，姜黄素对[具体细菌名称]以及[具体细菌名称]与[具体细菌名称]的混合培养物也显示出抗生物膜活性。生物膜反应调节因子BfmR的分子对接表明，类黄酮与BfmR的结合效力与抗生物膜效力相关。此外，在无细胞毒性的[具体动物模型名称]模型中，姜黄素处理降低了[具体细菌名称]的毒力。该研究表明姜黄素和其他类黄酮具有控制[具体细菌名称]生物膜形成及其毒力的潜力。
