LiaFSR和BsrXRS系统有助于分离株中的胆盐抗性。
The LiaFSR and BsrXRS Systems Contribute to Bile Salt Resistance in Isolates.
作者信息
Zhou Luoxiong, Wang Lihong, Tian Ping, Bao Tingting, Li Lianbin, Zhao Xin
机构信息
College of Animal Science and Technology, Northwest A&F University, Xianyang, China.
Department of Animal Science, McGill University, Montreal, QC, Canada.
出版信息
Front Microbiol. 2019 May 10;10:1048. doi: 10.3389/fmicb.2019.01048. eCollection 2019.
Two-component systems (TCSs) are dominant regulating components in bacteria for responding to environmental stimuli. However, little information is available on how TCSs in respond to bile salts - an important environmental stimulus for intestinal bacteria. In this study, the gene expression of 2 TCSs, BsrXRS and LiaFSR, was positively correlated with survival rates of different isolates during exposure to ox gall. Moreover, gene disruptions of , , , and significantly reduced the survival rates of in the presence of ox gall. Finally, EMSA results indicated that BsrR functioned as a transcription regulator for expression of its own gene as well as lipoate-protein ligase A (). Additional 27 potential target genes by BsrR were revealed through analyses. These findings suggest that BsrXRS and LiaFSR systems play important roles in bile salt resistance in .
双组分系统（TCSs）是细菌中应对环境刺激的主要调节成分。然而，关于肠道细菌中的双组分系统如何响应胆盐（一种对肠道细菌重要的环境刺激物）的信息却很少。在本研究中，两种双组分系统BsrXRS和LiaFSR的基因表达与不同分离株在暴露于牛胆汁期间的存活率呈正相关。此外，对bsrR、bsrS、liaF和liaS的基因破坏显著降低了在牛胆汁存在下的存活率。最后，电泳迁移率变动分析（EMSA）结果表明，BsrR作为其自身基因以及硫辛酸蛋白连接酶A（LipA）表达的转录调节因子发挥作用。通过RNA测序分析揭示了另外27个BsrR的潜在靶基因。这些发现表明，BsrXRS和LiaFSR系统在对胆盐的抗性中发挥重要作用。
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