DNA编码大环肽文库

DNA-Encoded Macrocyclic Peptide Library.

作者信息

Zhu Zhengrong, Shaginian Alex, Grady La Shadric C, Davie Christopher P, Lind Kenneth, Pal Sandeep, Thansandote Praew, Simpson Graham L

机构信息

GlaxoSmithKline, Cambridge, MA, USA.

GlaxoSmithKline, Stevenage, UK.

出版信息

Methods Mol Biol. 2019;2001:273-284. doi: 10.1007/978-1-4939-9504-2_12.

DOI:10.1007/978-1-4939-9504-2_12

PMID:31134575

Abstract

DNA-encoded library technology (ELT) is a cutting-edge enabling technology platform for drug discovery. Here we describe how to design and synthesize a macrocyclic DNA-encoded library; how to perform selection, sequencing, and data analysis to identify potential active peptides; and how to synthesize off-DNA peptides to confirm activity. This approach provides an effective tool for pharmaceutical research based on peptides.

摘要

DNA编码文库技术（ELT）是一种用于药物发现的前沿赋能技术平台。在此，我们描述如何设计和合成大环DNA编码文库；如何进行筛选、测序和数据分析以鉴定潜在的活性肽；以及如何合成非DNA肽以确认活性。这种方法为基于肽的药物研究提供了一种有效的工具。