淋病奈瑟菌提取物中硫代葡萄糖苷酶活性的指征

Indication of thioglucosidase activity in extracts of Neisseria gonorrhoeae.

作者信息

Goldner M, Nairn C A, Patel P V, Martin P M, Tan E L, Parsons N J, Smith H

出版信息

Ann Inst Pasteur Microbiol. 1987 May-Jun;138(3):325-32. doi: 10.1016/0769-2609(87)90120-7.

Abstract

The recent demonstration of a host-derived inducer of gonococcal resistance to complement-mediated killing by human serum in purified serum and red blood cell fractions, which contained small glucopeptides with cysteine as one of the constituent amino acids, prompted an investigation of Neisseria gonorrhoeae for thioglucosidase activity. This involved an examination of supernatants of sonicated gonococci for thioglucoside hydrolysis by following, spectroscopically, the hydrolysis of 6-purine beta-D-glucothiopyranoside to 6-mercaptopurine and glucose. Extracts of N. gonorrhoeae were found to hydrolyse this thioglucoside. A substantial activity was present in a fraction with the same order of molecular weight as plant thioglucosidases. This led us to suspect the presence of a thioglucosidase in N. gonorrhoeae.

摘要

最近在纯化的血清和红细胞组分中发现了一种宿主来源的诱导物，它能使淋病奈瑟菌对人血清介导的补体杀伤产生抗性，这些组分含有以半胱氨酸作为组成氨基酸之一的小糖肽，这促使人们对淋病奈瑟菌的硫代葡萄糖苷酶活性进行研究。这包括通过光谱法跟踪6-嘌呤β-D-硫代葡萄糖苷水解为6-巯基嘌呤和葡萄糖，来检测超声处理过的淋球菌上清液中硫代葡萄糖苷的水解情况。发现淋病奈瑟菌的提取物能水解这种硫代葡萄糖苷。在一个分子量与植物硫代葡萄糖苷酶相同量级的组分中存在大量活性。这使我们怀疑淋病奈瑟菌中存在硫代葡萄糖苷酶。