NanoBioMedical Centre, Adam Mickiewicz University, Poznań, Poland.
Department of Molecular Virology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
J Biomed Mater Res A. 2019 Oct;107(10):2244-2256. doi: 10.1002/jbm.a.36733. Epub 2019 Jun 10.
It is already known that cells respond strongly to topography and chemistry of 2D surfaces. In this work we study cell-material interactions; in particular, we investigated the attachment and alignment of SH-SY5Y cells of neuronal origin on grooved-patterns made from Silicon (Si) and Gold (Au). The Au-Si groove-pattern stimulated 93% of SH-SY5Y cells to differentiate into neuroblast-like type (N-type) in 2 days and outgrown neurites exhibited strong anisotropy along the grooves with 90% of cells having one or two neurites. In comparison, random distribution of morphology type, neurite number, and alignment were observed on control flat surfaces (Si and Au). We further show that designed Au-Si groove-patterns can be used to form reversed groove patterns on polycarolactone surface via soft lithography approach. Sixty-nine percentage of SH-SY5Y cells aligned along the obtained reversed groove patterns of the same dimensional characteristics to Si-Au grooves. In particular, this work demonstrated that the Au-Si grooves pattern stimulates neurite polarity, elongation, and morphological differentiation of neuroblastoma cells without any exogenous supply of growth factors or stimulants in just 2 days, which can lead to selective procedure of obtaining homologous population of neuron-like cells for future nerve regeneration therapies.
已知细胞对 2D 表面的形貌和化学性质有强烈的响应。在这项工作中，我们研究了细胞-材料的相互作用；特别是，我们研究了源于神经元的 SH-SY5Y 细胞在硅（Si）和金（Au）制成的凹槽图案上的黏附和排列。Au-Si 凹槽图案刺激 93%的 SH-SY5Y 细胞在 2 天内分化为类成神经细胞瘤（N 型），并且生长出的突起沿着凹槽具有强烈的各向异性，90%的细胞具有一个或两个突起。相比之下，在对照平面（Si 和 Au）上观察到形态类型、突起数量和排列的随机分布。我们进一步表明，通过软光刻方法可以在聚己内酯表面上形成与 Au-Si 凹槽图案相反的凹槽图案。69%的 SH-SY5Y 细胞沿着与 Si-Au 凹槽相同尺寸特征的获得的反转凹槽图案排列。特别是，这项工作表明，Au-Si 凹槽图案可以在短短 2 天内刺激神经母细胞瘤细胞的突起极性、伸长和形态分化，而无需任何外源性生长因子或刺激物的供应，这可能导致用于未来神经再生治疗的同源神经元样细胞的选择性获得程序。
