Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, China.
PLoS One. 2019 May 28;14(5):e0217018. doi: 10.1371/journal.pone.0217018. eCollection 2019.
Sustainable agriculture is an important global issue. The use of organic fertilizers can enhance crop yield and soil properties while restraining pests and diseases. The objective of this study was to assess the effects of long-term use of chemical and organic fertilizers on tea and rhizosphere soil properties in tea orchards. Inductively coupled plasma mass spectrometry (ICP-MS) and high-throughput sequencing technology analyses were used to investigate heavy metals content and bacterial composition in rhizosphere soils. Our results indicated that organic fertilizer treatment significantly decreased Cu, Pb and Cd contents in rhizosphere soil sample. The results also showed that treatment with organic fertilizer significantly decreased the contents of Cd, Pb and As in tea leaves. Furthermore, organic fertilizer significantly increased the amino acids content of tea and the pH of the soil. The use of organic fertilizer significantly increased in the relative abundance of Burkholderiales, Myxococcales, Streptomycetales, Nitrospirales, Ktedonobacterales, Acidobacteriales, Gemmatimonadales, and Solibacterales, and decreased the abundance of Pseudonocardiales, Frankiales, Rhizobiales, and Xanthomonadales. In conclusion, organic fertilizer can help to shape the microbial composition and recruit beneficial bacteria into the rhizosphere of tea, leading to improved tea quality and reduced heavy metals content in rhizosphere soil and tea leaves.
可持续农业是一个全球性的重要问题。使用有机肥可以提高作物产量和土壤性质，同时控制病虫害。本研究的目的是评估长期使用化肥和有机肥对茶园茶叶和根际土壤性质的影响。电感耦合等离子体质谱（ICP-MS）和高通量测序技术分析用于研究根际土壤中重金属含量和细菌组成。我们的结果表明，有机肥处理显著降低了根际土壤样品中 Cu、Pb 和 Cd 的含量。结果还表明，有机肥处理显著降低了茶叶中 Cd、Pb 和 As 的含量。此外，有机肥显著增加了茶叶的氨基酸含量和土壤的 pH 值。有机肥的使用显著增加了伯克霍尔德氏菌目、粘球菌目、链霉菌目、硝化螺旋菌目、Ktedonobacterales、酸杆菌目、芽单胞菌目和浮霉菌目的相对丰度，降低了假诺卡氏菌目、弗兰克氏菌目、根瘤菌目和黄单胞菌目的丰度。总之，有机肥可以帮助塑造微生物组成，并将有益细菌招募到茶树的根际，从而提高茶叶品质，降低根际土壤和茶叶中重金属的含量。
