[PM2.5对变应性鼻炎大鼠模型鼻黏膜炎症因子及病理学的影响]
[Effect of PM2.5 on inflammatory factors and pathology of nasal mucosa in a rat model of allergic rhinitis].
作者信息
Guo Z Q, Zhao R W, Zhang R X, Deng C R, Dong W Y, Zhuang G S
机构信息
Department of Otorhinolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
出版信息
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019 May 7;54(5):362-366. doi: 10.3760/cma.j.issn.1673-0860.2019.05.009.
To investigate the effect of PM2.5 exposure on nasal inflammatory cytokines and nasal mucosal pathology in a rat model of allergic rhinitis (AR). Twenty-four healthy female SD rats were randomly divided into 3 groups by random number table method, with 8 rats in each group: normal control group (NC group), ovalbumin (OVA) induced AR model (AR group), and AR model group inhaled to PM2.5 at 200 μg/m(3), 3 h/d, for 30 d (ARE group). Nasal symptoms including sneezing, nasal rubs and nasal secretion were recorded. Levels of OVA specific IgE in serum, interleukin 6 (IL-6) and tumor necrosis factor-ɑ (TNF-ɑ) in nasal irrigating solution were measured by enzyme-linked immunosorbent assay (ELISA). The histopathological changes of nasal mucosa were observed by HE staining. SPSS 17.0 software was used to analyze the data. The number of sneezing, nasal rubs and the amount of nasal secretion in the ARE group were significantly higher than that in the AR group and the NC group (number of sneezing (15.38±1.68) times/15 min (11.63±1.13) times/15 min (1.75±0.71) times/15 min, number of nasal rubs (27.75±2.12) times/15 min (21.25±2.96) times/15 min (5.25±1.04) times/15 min, amount of nasal secretion (18.90±2.07) mg (13.83±1.81) mg (3.78±0.41) mg, values was 236.089, 224.139, 183.971, respectively, all 0.001). Statistically significant differences in OVA specific IgE, IL-6 and TNF-ɑ levels were observed in ARE group exceeded AR group and NC group (OVA specific IgE (25.42±2.51) ng/ml (18.07±1.07) ng/ml (1.47±0.26) ng/ml, IL-6 (123.30±18.86) pg/ml (63.49±11.29) pg/ml (16.87±3.29) pg/ml, TNF-ɑ (162.50±38.15) pg/ml (72.96±11.28) pg/ml (27.52±4.15) pg/ml, values was 481.604, 138.277, 63.938, respectively, all 0.001). HE staining showed that the nasal epithelial cells of NC group were intact and neatly arranged. Nasal mucosa epithelial cells were arranged in disorder in AR group, with tissue structure swelling. Partial shedding of nasal epithelial cells, mucosal basement membrane thickening, submucosal tissue interstitial edema, vasodilation and gland hyperplasia were found in ARE group. An increase inflammatory factors level such as IL-6 and TNF-ɑ aggravates pathological damage of nasal mucosa in a rat model of AR by exposure to PM2.5.
探讨细颗粒物（PM2.5）暴露对变应性鼻炎（AR）大鼠模型鼻黏膜炎症细胞因子及鼻黏膜病理学的影响。将24只健康雌性SD大鼠采用随机数字表法随机分为3组，每组8只：正常对照组（NC组）、卵清蛋白（OVA）诱导的AR模型组（AR组）、AR模型且吸入200μg/m³ PM2.5、每天3小时、共30天的组（ARE组）。记录包括打喷嚏、蹭鼻和鼻分泌物在内的鼻部症状。采用酶联免疫吸附测定（ELISA）法检测血清中OVA特异性IgE、鼻灌洗液中白细胞介素6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平。通过苏木精-伊红（HE）染色观察鼻黏膜的组织病理学变化。采用SPSS 17.0软件进行数据分析。ARE组的打喷嚏次数、蹭鼻次数和鼻分泌物量显著高于AR组和NC组（打喷嚏次数：（15.38±1.68）次/15分钟、（11.63±1.13）次/15分钟、（1.75±0.71）次/15分钟，蹭鼻次数：（27.75±2.12）次/15分钟、（21.25±2.96）次/15分钟、（5.25±1.04）次/15分钟，鼻分泌物量：（1,890±2.07）mg、（13.83±1.81）mg、（3.78±0.41）mg，F值分别为236.089、224.139、183.971，均P<0.001）。ARE组的OVA特异性IgE、IL-6和TNF-α水平与AR组和NC组相比差异有统计学意义（OVA特异性IgE：（25.42±2.51）ng/ml、（18.07±1.07）ng/ml、（1.47±0.26）ng/ml，IL-6：（123.30±18.86）pg/ml、（63.49±11.29）pg/ml、（16.87±3.29）pg/ml，TNF-α：（162.50±38.15）pg/ml、（72.96±11.28）pg/ml、（27.52±4.15）pg/ml，F值分别为481.604、138.277、63.938，均P<0.001）。HE染色显示，NC组鼻上皮细胞完整、排列整齐。AR组鼻黏膜上皮细胞排列紊乱，组织结构肿胀。ARE组可见鼻上皮细胞部分脱落、黏膜基底膜增厚、黏膜下组织间质水肿、血管扩张和腺体增生。暴露于PM2.5可使IL-6和TNF-α等炎症因子水平升高，加重AR大鼠模型鼻黏膜的病理损伤。
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