Manoharan K, Kinder D, Banerjee M R
Cancer Biochem Biophys. 1987 May;9(2):127-32.
A new E. coli DNA polymerase I directed nick translation assay was used for measuring 7,12-dimethylbenz[a]anthracene-induced in situ DNA damage and repair in mouse mammary epithelial cells in monolayer culture. The nick translation assay was capable of detecting a DMBA-dose dependent significant increase of DNA damage, and the same assay also allowed monitoring of the DNA repair activity provoked by DMBA treatment of the epithelial cells. This relatively simple method thus provides a rapid assay for carcinogen-induced in situ DNA damage and repair in an epithelial cell tumorigenic system.
一种新的大肠杆菌DNA聚合酶I介导的缺口平移测定法被用于测量7,12-二甲基苯并[a]蒽诱导的单层培养小鼠乳腺上皮细胞中的原位DNA损伤与修复。缺口平移测定法能够检测到DNA损伤随DMBA剂量增加而显著增加，并且该方法还能监测DMBA处理上皮细胞后引发的DNA修复活性。因此，这种相对简单的方法为上皮细胞致瘤系统中致癌物诱导的原位DNA损伤与修复提供了一种快速检测方法。
