Line J E, Brackett R E
Center for Food Safety and Quality Enhancement, University of Georgia Experiment Station, Griffin, Georgia 30223-1797 USA.
J Food Prot. 1995 Sep;58(9):1042-1044. doi: 10.4315/0362-028X-58.9.1042.
The aflatoxins are toxic and carcinogenic mycotoxins which contaminate a variety of foods and feeds. The bacterium Flavobacterium aurantiacum NRRL B-184 has been previously shown to be effective in degrading aflatoxin B) in liquid test medium as well as in several food types. The purpose of this study was to investigate the effects of an added nutrient source and added aflatoxin on the ability of F. aurantiacum to degrade aflatoxin B. Radioactively labeled aflatoxin B was added to test solutions containing cells in phosphate buffer or tryptic soy broth. Nonlabeled aflatoxin B was also added to similar flasks. Analysis of radioactive CO and water- and chloroform-soluble portions of the cell supernatant fluids revealed that neither added nutrients nor added nonlabeled toxin had a significant influence on the microbial transformation of aflatoxin B These results suggest that the microbial degradation of aflatoxin by F. aurantiacum is probably a mineralization phenomenon and not a co-metabolism. The ability of this organism to detoxify aflatoxin without the need for exogenous energy sources could be important to future endeavors attempting to use the organism, or the mechanisms responsible, in fermentation reactions.
黄曲霉毒素是污染各种食品和饲料的有毒且致癌的霉菌毒素。先前已证明橙色黄杆菌NRRL B - 184在液体测试培养基以及几种食品类型中对降解黄曲霉毒素B1有效。本研究的目的是调查添加营养源和添加黄曲霉毒素对橙色黄杆菌降解黄曲霉毒素B1能力的影响。将放射性标记的黄曲霉毒素B1添加到含有磷酸盐缓冲液或胰蛋白胨大豆肉汤中的细胞的测试溶液中。未标记的黄曲霉毒素B1也添加到类似的烧瓶中。对细胞上清液的放射性二氧化碳以及水和氯仿可溶部分的分析表明，添加的营养物和添加的未标记毒素均对黄曲霉毒素B1的微生物转化没有显著影响。这些结果表明，橙色黄杆菌对黄曲霉毒素的微生物降解可能是一种矿化现象，而不是共代谢。这种生物体无需外源能源即可解毒黄曲霉毒素的能力对于未来尝试在发酵反应中使用该生物体或其负责机制的努力可能很重要。
