Puchois P, Luley C, Alaupovic P
Clin Chem. 1987 Sep;33(9):1597-602.
Because lipoproteins containing apolipoprotein A (ApoA-I + ApoA-II) or apolipoprotein B (ApoB) seem to exert opposite effects as risk factors for coronary heart disease, we decided to determine the separability of these two major plasma lipoproteins by procedures originally designed to separate high-density from low- and very-low-density lipoproteins. The presumably ApoB-free lipoproteins isolated from normal plasma by (a) ultracentrifugation at d = 1.063; precipitation with (b) heparin-Mn2+ or (c) phosphotungstate-Mg2+; or (d) immunoprecipitation with antibodies to ApoB were characterized by quantifying cholesterol and apolipoproteins A-I, A-II, B, C-II, C-III, D, E, F, and Lp(a). ApoA- and ApoB-containing lipoproteins were completely separated only by immunoprecipitation with antibodies to ApoB. The ApoB-containing lipoproteins isolated by other procedures always contained 4% to 20% of total plasma ApoA-I and differed substantially from one another with respect to the content of some of the minor apolipoproteins. Measuring apolipoproteins was more reliable than measuring cholesterol for monitoring this separation and for expressing the concentrations of ApoA- and ApoB-containing lipoproteins.
由于含有载脂蛋白A（载脂蛋白A-I + 载脂蛋白A-II）或载脂蛋白B（载脂蛋白B）的脂蛋白作为冠心病的危险因素似乎发挥着相反的作用，我们决定通过最初设计用于分离高密度脂蛋白与低密度脂蛋白和极低密度脂蛋白的方法来确定这两种主要血浆脂蛋白的可分离性。通过以下方法从正常血浆中分离出可能不含载脂蛋白B的脂蛋白：（a）在密度d = 1.063下超速离心；（b）用肝素-Mn2+或（c）磷钨酸-Mg2+沉淀；或（d）用抗载脂蛋白B抗体进行免疫沉淀，通过对胆固醇以及载脂蛋白A-I、A-II、B、C-II、C-III、D、E、F和脂蛋白（a）进行定量来对其进行表征。仅通过用抗载脂蛋白B抗体进行免疫沉淀才能完全分离含载脂蛋白A和含载脂蛋白B的脂蛋白。通过其他方法分离出的含载脂蛋白B的脂蛋白总是含有总血浆载脂蛋白A-I的4%至20%，并且在一些次要载脂蛋白的含量方面彼此有很大差异。在监测这种分离以及表示含载脂蛋白A和含载脂蛋白B的脂蛋白浓度时，测量载脂蛋白比测量胆固醇更可靠。
