Luc G, Fievet C, Arveiler D, Evans A E, Bard J M, Cambien F, Fruchart J C, Ducimetiere P
Department of Atherosclerosis, Institut Pasteur de Lille, France.
J Lipid Res. 1996 Mar;37(3):508-17.
Apolipoprotein (apo) C-III and apoE are components of two major classes of plasma lipoproteins, i.e., apoB- and non-apoB-containing lipoproteins. To analyze the relationship between the distribution of apoC-III and apoE among lipoproteins and coronary heart disease, we compared the distribution of these two apolipoproteins in survivors of myocardial infarction (MI) and control subjects, within and between populations at contrasting risk for MI. ApoC-III and apoE concentrations were determined in plasma devoid of apoB-containing lipoproteins by immunoprecipitation using a specific anti-apoB antiserum. These assays referred to apoC-III-Lp non-B and apoE-Lp non-B, respectively. By examining the difference with total plasma apoC-III and apoE levels, we calculated apoC-III and apoE in apoB-containing lipoproteins (apoCIII-LpB and apoE-LpB, respectively). These determinations were performed in control subjects and in survivors of MI, all males aged 25 to 64 years. They were recruited in Northern Ireland and France, countries characterized by a large difference in the incidence of coronary heart disease. In univariate analysis, apoCIII-LpB appeared significantly higher and the apoC-III ratio (apoC-III-Lp non-B/apoC-III-LpB) significantly lower in MI survivors than in control subjects in both countries. ApoE-LpB was higher in MI survivors than in control subjects in Northern Ireland but not in France. The two French and Irish control populations differed for apoC-ILL-Lp non-B, apoC-III ratio, and apoE ratio, which were higher in France than in Northern Ireland, and for apoC-III-LpB, apoE, and apoE-LpB, which were lower. Multivariate analysis showed that no parameter involving apoC-III and apoE was more discriminatory than HDL-cholesterol, cholesterol, and triglycerides or apoA-I, apoB, and triglycerides between controls and MI subjects. In contrast, the apoC-III ratio was a better discriminatory parameter between the two control populations than the listed parameters. The differences between the two control populations are of particular interest because they are not biased by the presence of disease or the large difference of the incidence in coronary heart disease between the two countries. It is suggested that the distribution of apoC-III among lipoproteins may play a role in the different susceptibility of the two populations to the atherogenic process.
载脂蛋白（apo）C-III和apoE是血浆中两类主要脂蛋白的组成成分，即含apoB和不含apoB的脂蛋白。为分析脂蛋白中apoC-III和apoE的分布与冠心病之间的关系，我们比较了心肌梗死（MI）幸存者和对照受试者中这两种载脂蛋白的分布情况，这些受试者来自心肌梗死风险不同的人群内部及不同人群之间。使用特异性抗apoB抗血清通过免疫沉淀法测定不含含apoB脂蛋白的血浆中apoC-III和apoE的浓度。这些检测分别针对apoC-III-Lp非B和apoE-Lp非B。通过检查与总血浆apoC-III和apoE水平的差异，我们计算了含apoB脂蛋白中的apoC-III和apoE（分别为apoCIII-LpB和apoE-LpB）。这些测定在年龄为25至64岁的男性对照受试者和MI幸存者中进行。他们分别在北爱尔兰和法国招募，这两个国家冠心病发病率差异很大。在单变量分析中，在这两个国家，MI幸存者中的apoCIII-LpB均显著高于对照受试者，而apoC-III比率（apoC-III-Lp非B/apoC-III-LpB）则显著低于对照受试者。在北爱尔兰，MI幸存者中的apoE-LpB高于对照受试者，但在法国并非如此。法国和爱尔兰的两个对照人群在apoC-III-Lp非B、apoC-III比率和apoE比率方面存在差异，法国的这些指标高于北爱尔兰；在apoC-III-LpB、apoE和apoE-LpB方面也存在差异，法国的这些指标低于北爱尔兰。多变量分析表明，在对照受试者和MI受试者之间，没有涉及apoC-III和apoE的参数比高密度脂蛋白胆固醇、胆固醇、甘油三酯或apoA-I、apoB和甘油三酯更具鉴别力。相比之下，apoC-III比率在两个对照人群之间是比上述参数更好的鉴别参数。两个对照人群之间的差异特别值得关注，因为它们不受疾病存在或两国冠心病发病率巨大差异的影响。有人提出，脂蛋白中apoC-III的分布可能在这两个人群对动脉粥样硬化过程的不同易感性中起作用。
