Atanasova Petia, Atanasov Vladimir, Wittum Lisa, Southan Alexander, Choi Eunjin, Wege Christina, Kerres Jochen, Eiben Sabine, Bill Joachim
Institute for Materials Science, University of Stuttgart, Heisenbergstr. 3, 70569 Stuttgart, Germany.
Institute of Chemical Process Engineering, University of Stuttgart, Böblinger Straße 78, 70199 Stuttgart, Germany.
Nanomaterials (Basel). 2019 May 24;9(5):800. doi: 10.3390/nano9050800.
The robust, anisotropic tobacco mosaic virus (TMV) provides a monodisperse particle size and defined surface chemistry. Owing to these properties, it became an excellent bio-template for the synthesis of diverse nanostructured organic/inorganic functional materials. For selective mineralization of the bio-template, specific functional groups were introduced by means of different genetically encoded amino acids or peptide sequences into the polar virus surface. An alternative approach for TMV surface functionalization is chemical coupling of organic molecules. To achieve mineralization control in this work, we developed a synthetic strategy to manipulate the surface hydrophilicity of the virus through covalent coupling of polymer molecules. Three different types of polymers, namely the perfluorinated (poly(pentafluorostyrene) (PFS)), the thermo-responsive poly(propylene glycol) acrylate (PPGA), and the block-copolymer polyethylene-block-poly(ethylene glycol) were examined. We have demonstrated that covalent attachment of hydrophobic polymer molecules with proper features retains the integrity of the virus structure. In addition, it was found that the degree of the virus hydrophobicity, examined via a ZnS mineralization test, could be tuned by the polymer properties.
坚固的各向异性烟草花叶病毒（TMV）具有单分散的粒径和确定的表面化学性质。由于这些特性，它成为合成各种纳米结构有机/无机功能材料的理想生物模板。为了使生物模板进行选择性矿化，通过不同的基因编码氨基酸或肽序列将特定的官能团引入到极性病毒表面。TMV表面功能化的另一种方法是有机分子的化学偶联。为了在这项工作中实现矿化控制，我们开发了一种合成策略，通过聚合物分子的共价偶联来操纵病毒的表面亲水性。研究了三种不同类型的聚合物，即全氟聚合物（聚（五氟苯乙烯）（PFS））、热响应性聚（丙二醇）丙烯酸酯（PPGA）和嵌段共聚物聚乙烯-嵌段-聚（乙二醇）。我们已经证明，具有适当特性的疏水性聚合物分子的共价连接保留了病毒结构的完整性。此外，通过硫化锌矿化试验发现，病毒的疏水性程度可以通过聚合物性质进行调节。
