用于通过厚度剪切模式声学方法检测白血病细胞的DNA适配体
DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method.
作者信息
Poturnayová Alexandra, Buríková Monika, Bízik Jozef, Hianik Tibor
机构信息
Department of Nuclear Physics and Biophysics Faculty of Mathematics, Physics and Informatics, Comenius University Mlynská dolina F1, 842 48, Bratislava, Slovakia.
Institute of Animal Biochemistry and Genetics, Center of Biosciences SAS Dúbravská cesta 9, 840 05, Bratislava, Slovakia.
出版信息
Chemphyschem. 2019 Feb 18;20(4):545-554. doi: 10.1002/cphc.201801126. Epub 2019 Feb 6.
By using the thickness shear mode acoustics method (TSM) and single-molecule force spectroscopy (SMFS) we studied the interactions between DNA aptamers (sgc8c) specific to the protein tyrosine kinase 7 (PTK7), which is localized in the membranes of leukemia lymphoblastics (MOLT-4), and lymphocyte (Jurkat) cell lines, as well with PTK7-negative U266 myeloid leukemia cells. The TSM method allowed the development of a highly sensitive, label-free biosensor for the detection leukemia cells with a limit of detection of (195±20) cells/mL. SMFS approved the high selectivity of the sgc8c aptamers to the PTK7 receptors at the cell surface and allowed determining the binding probability of the aptamers to the PTK7 receptors at different cell lines.
通过使用厚度剪切模式声学方法（TSM）和单分子力谱法（SMFS），我们研究了白血病淋巴母细胞（MOLT-4）和淋巴细胞（Jurkat）细胞系膜中定位的蛋白酪氨酸激酶7（PTK7）特异性DNA适配体（sgc8c）与PTK7阴性的U266髓系白血病细胞之间的相互作用。TSM方法使得能够开发出一种高灵敏度、无标记的生物传感器，用于检测白血病细胞，检测限为（195±20）个细胞/毫升。SMFS证实了sgc8c适配体对细胞表面PTK7受体具有高选择性，并能够确定适配体与不同细胞系中PTK7受体的结合概率。
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