Departamento de Bioquímica, Instituto de Química - Universidade de São Paulo, São Paulo, SP, Brazil.
Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Adv Exp Med Biol. 2019;1127:21-35. doi: 10.1007/978-3-030-11488-6_2.
Lipids containing unsaturated doubles bonds are oxidized by enzymatic and non-enzymatic mechanisms yielding hydroperoxides and hydroxides as primary products.This process has been implicated in physiological and pathological mechanisms. Thus, precise characterization and quantification of lipid oxidation products in biological samples can provide important mechanistic insights. In this context, the use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to characterize the primary products of polyunsaturated fatty acids, like hydroxides and hydroperoxides, is a sensitive, specific and versatile tool. Here we will focus on the characterization and specific analysis of hydroxy and hydroperoxy regioisomers of linoleic acid, arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid by LC-MS/MS.
含不饱和双键的脂质可通过酶促和非酶促机制被氧化，生成过氧化物和氢氧化物等初级产物。这一过程与生理和病理机制有关。因此，对生物样本中脂质氧化产物进行精确的定性和定量分析可以提供重要的机制见解。在这种情况下，使用液相色谱-串联质谱（LC-MS/MS）来表征多不饱和脂肪酸（如过氧化物和氢氧化物）的初级产物是一种灵敏、特异和通用的工具。在这里，我们将重点介绍通过 LC-MS/MS 对亚油酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸的羟基和过氧基位置异构体进行的定性和特异性分析。
