Masoodi Mojgan, Mir Adnan A, Petasis Nicos A, Serhan Charles N, Nicolaou Anna
School of Pharmacy, University of Bradford, Richmond Road, Bradford, UK.
Rapid Commun Mass Spectrom. 2008;22(2):75-83. doi: 10.1002/rcm.3331.
Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) exhibit a range of tissue- and cell-specific activities in many physiological and pathological processes. Electrospray ionisation tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI-MS/MS assay for the simultaneous analysis of twenty mono- and poly-hydroxy-fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1-100 pg/microL, whilst the limits of detection and quantitation were 10-20 and 20-50 pg, respectively. The recovery of the extraction methodology varied from 76-122% depending on the metabolite. This system is useful for profiling a range of biochemically related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxyeicosapentaenoic and hydroxydocosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease.
源自多不饱和脂肪酸（PUFA）的生物活性脂质介质在许多生理和病理过程中表现出一系列组织和细胞特异性活性。液相色谱-电喷雾电离串联质谱联用技术（LC/ESI-MS/MS）是一种用于脂质介质定性和定量分析的灵敏且通用的分析方法。在此，我们展示了一种LC/ESI-MS/MS检测方法，用于同时分析亚油酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸的二十种单羟基和多羟基脂肪酸衍生物。该检测方法在1-100 pg/μL的浓度范围内呈线性，而检测限和定量限分别为10-20 pg和20-50 pg。提取方法的回收率因代谢物而异，在76%-122%之间。该系统有助于对一系列生物化学相关的强效介质进行分析，包括新发现的消退素和保护素，以及它们的前体羟基二十碳五烯酸和羟基二十二碳六烯酸，从而增进我们对PUFA在健康和疾病中作用的理解。
