a College of Medicine and Public Health, Flinders University , Bedford Park , Australia.
b Department of Biology, Laurentian University , Sudbury , Canada.
Int J Radiat Biol. 2019 Aug;95(8):1072-1084. doi: 10.1080/09553002.2019.1625459. Epub 2019 Jun 12.
Diagnostic radiation is an important part of patient care in the Intensive Care Unit; however, there is little data on the acute effects of exposure to these doses. We investigated pulmonary and splenic response 30 minutes, 4 hours or 24 hours after exposure to 2 mGy, 20 mGy, 200 mGy or 4 Gy whole-body X-radiation in a Sprague Dawley rat model. Lung injury was assessed via respiratory mechanics, pulmonary edema, cellular, and proteinaceous fluid infiltrate and protein expression of oxidative stress markers. The radiation effect on the spleen was determined via proliferation, apoptosis and protein expression of oxidative stress markers. All measurements of the lung did not differ from sham animals except for an increase in catalase after high dose exposure. Stimulated splenocyte proliferation increased after sham and low dose exposure, did not change after 200 mGy exposure and was significantly lower after 4 Gy exposure. The number of apoptotic cells increased 4 hours after 4 Gy exposure. There were fewer apoptotic cells after low dose exposure compared to sham. Both catalase and MnSOD were increased after 4 Gy exposure. There was no measured effect on pulmonary function while there was an impact to the spleen after low and high dose exposure.
诊断辐射是重症监护病房患者治疗的重要组成部分；然而，关于暴露于这些剂量的急性影响的数据很少。我们在 Sprague Dawley 大鼠模型中研究了暴露于 2mGy、20mGy、200mGy 或 4Gy 全身 X 射线后 30 分钟、4 小时或 24 小时时肺部和脾脏的反应。通过呼吸力学、肺水肿、细胞和蛋白质液体浸润以及氧化应激标志物的蛋白表达来评估肺损伤。通过增殖、细胞凋亡和氧化应激标志物的蛋白表达来确定辐射对脾脏的影响。除了高剂量暴露后过氧化氢酶增加外，肺的所有测量值与假手术动物均无差异。刺激脾细胞增殖在假手术和低剂量暴露后增加，在 200mGy 暴露后没有变化，在 4Gy 暴露后明显降低。4Gy 暴露 4 小时后凋亡细胞数量增加。与假手术相比，低剂量暴露后凋亡细胞减少。4Gy 暴露后均增加了过氧化氢酶和 MnSOD。虽然低剂量和高剂量暴露对脾脏有影响，但对肺功能没有影响。
