Mohye El-Din Amal A, Abdelrazzak Abdelrazek B, Ahmed Moustafa T, El-Missiry Mohamed A
1 Department of Physics, Faculty of Science, Mansoura University , Mansoura , Egypt.
2 Spectroscopy Department, Physics Research Division, National Research Centre , Cairo , Egypt.
Br J Radiol. 2017 Dec;90(1080):20170278. doi: 10.1259/bjr.20170278. Epub 2017 Sep 20.
To investigate the radiation-induced abscopal effect in terms of oxidative stress, apoptosis and DNA damage in the spleen cells following cranial X-rays irradiation of rats.
Rats were cranially irradiated using 2 Gy X-rays. Another group was whole-body irradiated with 2 Gy X-rays and a third group was exposed to scattered radiation (measured to be 3 mGy). 24 hours following irradiation, sections from the spleen of the rats were dissected as well as plasma samples. The samples were examined for the desired endpoints.
The cranially irradiated animals showed a significant increase in the levels of glutathione, superoxide dismutase and catalase with no significant change in the lipid peroxidation product in the spleen cells with a significant increase in the C-reactive protein level the plasma. Apoptotic cell death in the spleen cells was demonstrated as indicated by the decrease of Bcl-2; the increase of p53, Bax, caspase-3 and caspase-8 and induction of DNA damage in the spleen in both of the cranially irradiated rats and whole body exposed rats. The exposure to 3 mGy scattered radiation increased the plasma level of C-RP and also induced apoptosis in the spleen cells.
Cranial irradiation-induced abscopal effect in distant spleen cells. Very low doses of radiation can induce apoptosis in the spleen cells. Advances in knowledge: This paper provides an evidence on the incidence of radiation abscopal effect. Also, the results shed light of the effect very low doses of radiation as low as 3 mGy.
研究大鼠头部X线照射后，脾脏细胞中氧化应激、细胞凋亡和DNA损伤方面的辐射诱导远隔效应。
用2 Gy X线对大鼠进行头部照射。另一组用2 Gy X线进行全身照射，第三组暴露于散射辐射（测量值为3 mGy）。照射后24小时，解剖大鼠脾脏组织切片并采集血浆样本。对样本进行所需终点指标的检测。
头部照射的动物脾脏细胞中谷胱甘肽、超氧化物歧化酶和过氧化氢酶水平显著升高，脂质过氧化产物无显著变化，血浆中C反应蛋白水平显著升高。脾脏细胞凋亡性细胞死亡表现为Bcl-2减少；p53、Bax、半胱天冬酶-3和半胱天冬酶-8增加，头部照射大鼠和全身暴露大鼠的脾脏均出现DNA损伤。暴露于3 mGy散射辐射会增加血浆C-RP水平，也会诱导脾脏细胞凋亡。
头部照射可诱导远处脾脏细胞产生远隔效应。极低剂量辐射可诱导脾脏细胞凋亡。知识进展：本文提供了辐射远隔效应发生率的证据。此外，结果揭示了低至3 mGy的极低剂量辐射的效应。
