State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Xianning Vocational Technical College, Xianning 437100, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):2972-2978. doi: 10.1099/ijsem.0.003477.
A Gram-stain-negative, single polar flagellum bacterium, WZY27, was isolated from rhizospheric soil of plants. The results of phylogenetic analysis based on 16S rRNA gene sequences showed that this strain is closely related to DSM 7418 (97.2 % similarity), KCTC 2883 (97.1 %) and JCM 17074 (97.0 %). The genomic average nucleotide identity values between strain WZY27 and the above three strains were 75.3, 73.2 and 75.4 %, and the DNA-DNA hybridization values were 19.1 , 20.1 and 20.9 %, respectively. The major fatty acids (>5 %) of strain WZY27 were summed feature 8 (C 7/C 6), C, C 2-OH and C 7 11-methyl. The predominant respiratory quinone and polyamine were ubiquinone Q-10 and homospermidine, respectively. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, glycolipids, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 68.4 mol%. Based on the results of genotypic, chemotaxonomic and phenotypic characterization, strain WZY27 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WZY27 (=KCTC 62523=CCTCC AB 2018056).
一株革兰氏阴性、单极鞭毛细菌，WZY27，从 植物的根际土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析结果表明，该菌株与 DSM 7418（97.2%相似性）、KCTC 2883（97.1%相似性）和 JCM 17074（97.0%相似性）密切相关。菌株 WZY27 与上述三株菌的基因组平均核苷酸同一性值分别为 75.3%、73.2%和 75.4%，DNA-DNA 杂交值分别为 19.1%、20.1%和 20.9%。菌株 WZY27 的主要脂肪酸（>5%）为 summed feature 8 (C 7/C 6)、C 16:0、C 2-OH 和 C 7 11-methyl。主要呼吸醌和多胺分别为泛醌 Q-10 和腐胺。极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂、糖脂、磷脂酰胆碱和神经酰胺糖脂。基因组 DNA 的 G+C 含量为 68.4mol%。基于基因型、化学分类和表型特征的结果，菌株 WZY27 代表了一个新的属 种，建议命名为 sp. nov.。模式菌株为 WZY27（=KCTC 62523=CCTCC AB 2018056）。
