Yan Zheng-Fei, Lin Pei, Won Kyung-Hwa, Li Chang-Tian, Park GyungSoo, Chin ByungSun, Kook MooChang, Wang Qi-Jun, Yi Tae-Hoo
College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Int J Syst Evol Microbiol. 2018 Feb;68(2):681-686. doi: 10.1099/ijsem.0.002566.
A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 5.0-9.0 (optimum 7.0) and in the presence of 0-2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61 were identified as Sphingomonas ginsengisoli KCTC 12630 (97.9 %), Sphingomonas jaspsi DSM 18422 (97.8 %), Sphingomonas astaxanthinifaciens NBRC 102146 (97.4 %), Sphingomonassediminicola KCTC 12629 (97.2 %), 'Sphingomonas swuensis' KCTC 12336 (97.1 %) and Sphingomonas daechungensis KCTC 23718 (96.9 %). The isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids were C16 : 0, C17 : 1ω6c, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and summed feature 7 (C18 : 1ω7c, C18 : 1ω9t and/or C18 : 1ω12t). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified lipid, one unidentified phospholipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The polyamine was homospermidine. The DNA G+C content of strain THG-T61 was 65.6 mol%. The DNA-DNA relatedness values between strain THG-T61 and its closest reference strains were less than 49.2 %, which is lower than the threshold value of 70 %. Therefore, strain THG-T61 represents a novel species of the genus Sphingomonas, for which the name Sphingomonas rhizophila sp. nov. is proposed. The type strain is THG-T61 (=KACC 19189=CCTCC AB 2016245).
从木槿根际分离出一株革兰氏阴性、需氧、无运动性、杆状、过氧化氢酶阳性和氧化酶阳性细菌（THG-T61）。该菌在10-37 °C（最适温度25-30 °C）、pH 5.0-9.0（最适pH 7.0）以及0-2.0 % NaCl（最适无NaCl添加）条件下生长。基于16S rRNA基因序列分析，菌株THG-T61最近的系统发育邻种被鉴定为人参鞘氨醇单胞菌KCTC 12630（相似度97.9 %）、雅氏鞘氨醇单胞菌DSM 18422（相似度97.8 %）、虾青素鞘氨醇单胞菌NBRC 102146（相似度97.4 %）、沉积鞘氨醇单胞菌KCTC 12629（相似度97.2 %）、“四川鞘氨醇单胞菌”KCTC 12336（相似度97.1 %）和大川鞘氨醇单胞菌KCTC 23718（相似度96.9 %）。类异戊二烯醌为泛醌-10（Q-10）。主要脂肪酸为C16 : 0、C17 : 1ω6c、特征性总和4（异-C15 : 0 2-OH和/或C16 : 1ω7c）和特征性总和7（C18 : 1ω7c、C18 : 1ω9t和/或C18 : 1ω12t）。极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、鞘糖脂、一种未鉴定的脂类、一种未鉴定的磷脂、一种未鉴定的糖脂和一种未鉴定的磷酸糖脂。多胺为高亚精胺。菌株THG-T61的DNA G+C含量为65.6 mol%。菌株THG-T61与其最接近的参考菌株之间的DNA-DNA相关性值低于49.2 %，低于70 %的阈值。因此，菌株THG-T61代表鞘氨醇单胞菌属的一个新物种，提议将其命名为根际鞘氨醇单胞菌（Sphingomonas rhizophila sp. nov.）。模式菌株为THG-T61（=KACC 19189=CCTCC AB 2016245）。
