Hellstern P, Miyashita C, Köhler M, von Blohn G, Kiehl R, Biro G, Schwerdt H, Wenzel E
Haemostasis. 1987;17(4):173-81. doi: 10.1159/000215740.
Factor VIII coagulant antigen (FVIII:Ag) and FVIII coagulant activity (FVIII:C) were measured in 102 healthy individuals, in 5 hemophilia A carriers and in 21 hemophilia A patients before and after infusion of heat-treated high-purity FVIII concentrates. Factor VIII:Ag was determined by a solid-phase micro enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies and by a conventional solid-phase immunoradiometric assay (IRMA). Factor VIII:C was assessed using a one-stage assay. The micro ELISA was decidedly more precise than the IRMA. There was a close correlation between the results obtained by the three assays in the plasma of healthy subjects and in hemophilia A carriers. After transfusion of FVIII concentrates to hemophilia A patients, the FVIII:Ag recoveries were significantly lower than the FVIII:C recoveries and the biological half-life of FVIII:Ag was significantly shorter than for FVIII:C. The calculated half-life of FVIII:C was longer than in any previous study.
在102名健康个体、5名甲型血友病携带者和21名甲型血友病患者中，于输注热处理高纯度凝血因子VIII浓缩物前后，测定了凝血因子VIII促凝抗原（FVIII:Ag）和凝血因子VIII促凝活性（FVIII:C）。凝血因子VIII:Ag通过使用单克隆抗体的固相微酶联免疫吸附测定法（ELISA）以及传统的固相免疫放射测定法（IRMA）进行测定。凝血因子VIII:C采用一期法进行评估。微ELISA比IRMA明显更精确。在健康受试者和甲型血友病携带者的血浆中，三种测定方法所获结果之间存在密切相关性。向甲型血友病患者输注凝血因子VIII浓缩物后，凝血因子VIII:Ag的回收率显著低于凝血因子VIII:C的回收率，且凝血因子VIII:Ag的生物学半衰期显著短于凝血因子VIII:C的生物学半衰期。计算得出的凝血因子VIII:C的半衰期比以往任何研究中的都要长。
