胃癌中 8q24.21 miRNA 簇表达和拷贝数变异的分析。
Analysis of 8q24.21 miRNA cluster expression and copy number variation in gastric cancer.
机构信息
Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, 04023-900, Brazil.
Disciplina de Gastroenterologia, Departamento de Medicina, Universidade Federal de São Paulo, São Paulo, 04040-002, Brazil.
出版信息
Future Med Chem. 2019 May;11(9):947-958. doi: 10.4155/fmc-2018-0477. Epub 2019 May 29.
To analyze gene expression and copy number of five miRNAs (, , , and ) localized in this chromosome region in gastric cancer (GC). 65 paired neoplastic and non-neoplastic specimens collected from GC patients and 20 non-neoplastic gastric tissues from cancer-free individuals were included in this study. The expression levels of the five miRNAs were accessed by real time qPCR and were correlated. , , and were upregulated in approximately 50% of GC tumors in relation to those of adjacent non-neoplastic tissues. expression was associated with gain of gene copies and was upregulated in adjacent non-neoplastic samples relative to external controls. The coexpression of the 8q24 miRNAs indicated the role of in the initiation of gastric cancer development.
分析位于该染色体区域的五个 miRNA（、、、和）在胃癌（GC）中的基因表达和拷贝数。本研究纳入了 65 对来自 GC 患者的肿瘤和非肿瘤标本和 20 例来自无癌个体的非肿瘤胃组织。通过实时 qPCR 检测五种 miRNA 的表达水平并进行相关性分析。与相邻非肿瘤组织相比，约 50%的 GC 肿瘤中上调了、、和。表达与基因拷贝数的增加有关，与外部对照相比，相邻非肿瘤样本中的表达上调。8q24miRNA 的共表达表明在胃癌发生发展的起始阶段发挥作用。
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