Zare Ali, Ahadi Alireza, Larki Pegah, Omrani Mir Davood, Zali Mohammad Reza, Alamdari Nasser Malekpour, Ghaedi Hamid
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Velenjak St., Shahid Chamran Highway, Tehran, Iran.
Urogenital Stem Cell Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mol Biol Rep. 2018 Dec;45(6):1587-1595. doi: 10.1007/s11033-018-4278-5. Epub 2018 Aug 31.
Gastric cancer (GC) is one of the leading types of malignancy worldwide, particularly in Asian populations. Although the exact molecular mechanism of GC development remains unknown, microRNA (miRNA) has recently been shown to be involved. The current study aims to investigate the expression levels of bioinformatically ranked miRNAs in gastric tissues. Using bioinformatics tools, we prioritized miRNAs thought to be implicated in GC. Furthermore, polyA-qPCR was used to validate bioinformatics findings in 40 GC, 31 normal gastric tissue (NG) and 45 gastric dysplasia (GD) samples. As identified by bioinformatics analysis, miR-335 was shown to be the top-ranked miRNA implicated in GC. Moreover, a significant downregulation of miR-335, miR-124, miR-218 and miR-484 was found in GC and GD compared to NG samples. We found bioinformatics to be an efficient approach to finding candidate miRNAs relevant to GC development. Finally, the findings show that downregulation of miRNAs such as miR-124 and miR-218 in gastric tissue can be a significant indicator for neoplastic transformation.
胃癌（GC）是全球主要的恶性肿瘤类型之一，在亚洲人群中尤为常见。尽管GC发生的确切分子机制尚不清楚，但最近研究表明微小RNA（miRNA）与之相关。本研究旨在调查生物信息学排名的miRNA在胃组织中的表达水平。我们使用生物信息学工具对认为与GC相关的miRNA进行了优先排序。此外，采用多聚腺苷酸定量聚合酶链反应（polyA-qPCR）在40例GC、31例正常胃组织（NG）和45例胃发育异常（GD）样本中验证生物信息学研究结果。生物信息学分析确定，miR-335是与GC相关的排名最靠前的miRNA。此外，与NG样本相比，在GC和GD样本中发现miR-335、miR-124、miR-218和miR-484显著下调。我们发现生物信息学是寻找与GC发生相关的候选miRNA的有效方法。最后，研究结果表明，胃组织中miR-124和miR-218等miRNA的下调可能是肿瘤转化的重要指标。
