Reddy M M, Grieco M H
Int J Immunopharmacol. 1987;9(4):483-8. doi: 10.1016/0192-0561(87)90023-3.
A monoclonal antibody (anti-Tac) that appears to bind the receptor for interleukin-2 (IL-2) was used to quantitate lymphocytes that express IL-2 receptors (IL-2R) in response to phytohemagglutinin (PHA) stimulation. 62 +/- 4% of the cells expressed IL-2R in response to PHA in twelve normal subjects compared to 22 +/- 4% in fourteen patients with acquired immune deficiency syndrome (AIDS) (P less than 0.001) and 50 +/- 6% in six patients with AIDS-related complex (P less than 0.1). There was no effect on IL-2R expression, when lymphocytes from seven controls were incubated with IL-2 (20 mu/ml) or thymosin fraction V (10 mu/ml) for 72 h. However, when the lymphocytes from seven patients with AIDS were incubated with IL-2, the IL-2R rose from 18 +/- 3% to 31 +/- 3% (P less than 0.005) and with a fraction V to 29 +/- 3% (P less than 0.001). In addition, IL-2 augmented the PHA-induced proliferative responses in patients with AIDS-related complex and AIDS and normal controls, whereas thymosin fraction V had no significant effect. Thymosin fraction V also enhanced the IL-2 production of PHA-stimulated mononuclear cells obtained from six patients with AIDS and six normal controls. These results suggest that both IL-2 and thymosin fraction V can modulate in vitro T-cell function in patients with AIDS.
一种似乎能与白细胞介素-2（IL-2）受体结合的单克隆抗体（抗Tac）被用于定量在植物血凝素（PHA）刺激下表达IL-2受体（IL-2R）的淋巴细胞。在12名正常受试者中，62±4%的细胞在PHA刺激下表达IL-2R，相比之下，14名获得性免疫缺陷综合征（AIDS）患者中这一比例为22±4%（P<0.001），6名艾滋病相关综合征患者中为50±6%（P<0.1）。当7名对照者的淋巴细胞与IL-2（20μg/ml）或胸腺素组分V（10μg/ml）孵育72小时时，对IL-2R表达没有影响。然而，当7名艾滋病患者的淋巴细胞与IL-2孵育时，IL-2R从18±3%升至31±3%（P<0.005），与胸腺素组分V孵育时升至29±3%（P<0.001）。此外，IL-2增强了艾滋病相关综合征患者、艾滋病患者和正常对照者中PHA诱导的增殖反应，而胸腺素组分V没有显著影响。胸腺素组分V还增强了从6名艾滋病患者和6名正常对照者获得的PHA刺激的单核细胞的IL-2产生。这些结果表明，IL-2和胸腺素组分V都可以在体外调节艾滋病患者的T细胞功能。
