Sztein M B, Simon G L, Parenti D M, Scheib R, Goldstein A L, Goodman R, DiGioia R, Paxton H, Skotnicki A B, Schulof R S
Clin Immunol Immunopathol. 1987 Jul;44(1):51-62. doi: 10.1016/0090-1229(87)90051-1.
The in vitro effects of thymosin fraction 5 (TF5) and lithium chloride (LiCl) on the ability of peripheral blood mononuclear cells (PBMC) obtained from 37 normal male donors and 33 male patients with AIDS-related complex (ARC) to respond to alloantigenic stimulation (mixed leukocyte reaction, MLR) and to produce interleukin 2 (IL-2) in response to mitogens were studied. TF5 significantly increased MLR responses in normal donors (P less than 0.01) and in a group of 33 ARC patients with depressed cellular immunity (P less than 0.05). Similar effects were observed when LiCl was added to the MLR assays in both the normal and the ARC patient groups. Furthermore, TF5 and LiCl exhibited additive immunoenhancing properties. In 10 normal donors TF5 enhanced phytohemaggutinin (PHA)-induced IL-2 production as well as IL-2 production in response to pokeweed mitogen (PWM) (P less than 0.02). TF5-mediated enhancement of IL-2 production by PBMC obtained from ARC patients was observed in response to both mitogens, i.e., PHA and PWM. Additionally, LiCl increased PHA-induced IL-2 production in both normal subjects and ARC patients. LiCl and TF5 together had an additive effect in the enhancement of IL-2 production in both groups of subjects. Our data extend previous observations regarding the immunoregulatory activities of TF5 and LiCl and provide evidence that PBMC obtained from ARC patients have the potential to respond in vitro to these agents. The significance of these findings is discussed.
研究了胸腺素5组分（TF5）和氯化锂（LiCl）对从37名正常男性供体和33名患艾滋病相关综合征（ARC）男性患者获取的外周血单个核细胞（PBMC），在同种异体抗原刺激（混合淋巴细胞反应，MLR）下的反应能力以及在有丝分裂原刺激下产生白细胞介素2（IL-2）能力的体外效应。TF5显著增加了正常供体（P<0.01）以及一组细胞免疫功能低下的33名ARC患者（P<0.05）的MLR反应。在正常和ARC患者组的MLR试验中加入LiCl时，观察到了类似的效应。此外，TF5和LiCl表现出相加的免疫增强特性。在10名正常供体中，TF5增强了植物血凝素（PHA）诱导的IL-2产生以及对商陆有丝分裂原（PWM）反应的IL-2产生（P<0.02）。观察到从ARC患者获取的PBMC在对两种有丝分裂原（即PHA和PWM）反应时，TF5介导的IL-2产生增强。此外，LiCl增加了正常受试者和ARC患者中PHA诱导的IL-2产生。LiCl和TF5共同对两组受试者IL-2产生的增强具有相加效应。我们的数据扩展了先前关于TF5和LiCl免疫调节活性的观察结果，并提供了证据表明从ARC患者获取的PBMC在体外有对这些药物作出反应的潜力。讨论了这些发现的意义。
