Lara C, Romero J M, Guerrero M G
J Bacteriol. 1987 Sep;169(9):4376-8. doi: 10.1128/jb.169.9.4376-4378.1987.
Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixation by DL-glyceraldehyde, with the effect of either compound being prevented by previously blocking ammonium assimilation. The results support the contention that nitrate utilization in cyanobacteria is regulated at the level of nitrate transport through the concerted action of ammonium assimilation and CO2 fixation.
在蓝细菌集胞藻的完整细胞中,已检测到硝酸盐的细胞内积累,这表明存在活跃的硝酸盐转运系统。添加铵盐或用DL-甘油醛选择性抑制二氧化碳固定,均能有效阻碍细胞积累硝酸盐的能力,而预先阻断铵同化作用可防止这两种化合物产生上述影响。这些结果支持了以下观点:蓝细菌中硝酸盐的利用是通过铵同化作用和二氧化碳固定的协同作用,在硝酸盐转运水平上受到调控的。