Ishida B Y, Frolich J, Fielding C J
J Lipid Res. 1987 Jul;28(7):778-86.
A quantitative solid phase immunoassay has been developed for the determination of the mass of electrophoretically separated prebeta apolipoprotein A-I (apoA-I) in human plasma. Conditions have been identified for the quantitative transfer and immunoblotting of the apolipoprotein in the absence of organic solvents or detergents. In normolipidemic plasma, the prebeta-migrating fraction of apoA-I represented 4.2 +/- 1.8% of total apoA-I (61 +/- 26 micrograms of apoA-I per ml of plasma). Significantly higher levels were found in hypercholesterolemia of genetic origin, in primary and secondary hypertriglyceridemia, and in congenital lecithin:cholesterol acyltransferase deficiency. In all cases prebeta-migrating apoA-I consisted in large part of low molecular weight lipoprotein species, compared to the size of the major, alpha-migrating apoA-I fraction.
已开发出一种定量固相免疫测定法，用于测定人血浆中经电泳分离的前β载脂蛋白A-I（apoA-I）的质量。已确定在不存在有机溶剂或去污剂的情况下进行载脂蛋白定量转移和免疫印迹的条件。在正常血脂血浆中，apoA-I的前β迁移部分占总apoA-I的4.2±1.8%（每毫升血浆中apoA-I为61±26微克）。在遗传性高胆固醇血症、原发性和继发性高甘油三酯血症以及先天性卵磷脂：胆固醇酰基转移酶缺乏症中发现了明显更高的水平。在所有情况下，与主要的α迁移apoA-I部分的大小相比，前β迁移的apoA-I在很大程度上由低分子量脂蛋白种类组成。
