a Department of Orthopedics, No. 89 hospital of Chinese People's Liberation Army , Weifang , Shandong , China.
b Department of hand and foot orthopedic, Weifang People's Hospital , Weifang , Shandong , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2139-2145. doi: 10.1080/21691401.2019.1613418.
Osteoarthritis (OA) is a common joint disease for which a safe and reliable treatment has yet to be developed. Here, we demonstrated the potential benefit of treatment with paeonol, a derivative of , in the treatment and prevention of OA. Chondrogenic cell line ATDC5 cells were cultured with IL-1β and the effects of paeonol were assessed through qRT-PCR, western blot analysis, MTT, ELISA, and NF-κB luciferase reporter gene assay. Our findings demonstrate a novel ability of paeonol to inhibit numerous factors of OA, including expressions of IL-6, TNF-α, NOX2, PTGS2, NUCB2/nesfatin-1, ICAM-1, VCAM-1, MMP-3/13, degradation of type II collagen, and NF-κB activation through the rescue of IκBα. Additionally, we assessed the effects of paeonol on cell viability to confirm its safety. These findings implicate a valuable potential role of paeonol in the treatment and prevention of OA.
骨关节炎（OA）是一种常见的关节疾病，尚未开发出安全可靠的治疗方法。在这里，我们证明了丹皮酚（PAE）治疗 OA 的潜在益处，PAE 是 的衍生物。将软骨细胞系 ATDC5 细胞与 IL-1β 共培养，并通过 qRT-PCR、western blot 分析、MTT、ELISA 和 NF-κB 荧光素酶报告基因检测评估 PAE 的作用。我们的研究结果表明，丹皮酚具有抑制 OA 多种因子的新能力，包括 IL-6、TNF-α、NOX2、PTGS2、NUCB2/nesfatin-1、ICAM-1、VCAM-1、MMP-3/13 的表达、II 型胶原蛋白的降解和 NF-κB 的激活，通过恢复 IκBα。此外，我们评估了丹皮酚对细胞活力的影响以确认其安全性。这些发现表明丹皮酚在 OA 的治疗和预防中有重要的潜在作用。
Artif Cells Nanomed Biotechnol. 2019-12
Artif Cells Nanomed Biotechnol. 2019-12
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