Kwiatkowska Dorota, Natonik-Białoń Sandra, Burian Agata
Department of Biophysics and Morphogenesis of Plants, University of Silesia in Katowice, Katowice, Poland.
Methods Mol Biol. 2019;1992:239-255. doi: 10.1007/978-1-4939-9469-4_16.
Complex geometry of plant organs and various types of organ surface deformation, including growth or hygroscopic movements, can be analyzed using sequential replica method. It enables obtaining a time-lapse series of high resolution images visualizing details of the examined surface and provides data sufficient for detailed computation of parameters characterizing surface deformation and geometry. Series of molds, made in dental polymer, representing the examined surface are used to obtain casts in epoxy resin or nail polish replicas, which are ready for microscopic examination, while the structure itself remains intact. Images obtained from the epoxy casts in scanning electron microscopy can be further used for 3D reconstruction and computation of local geometry. The sequential replica method is a universal method and can be applied to image complex shapes of a range of structures, like meristems, flowers, leaves, scarious bracts, or trichomes. Different plant species growing in various conditions can be studied.
利用连续复制法可以分析植物器官的复杂几何形状以及各种类型的器官表面变形，包括生长或吸湿运动。该方法能够获得一系列高分辨率的延时图像，可视化被检查表面的细节，并提供足够的数据用于详细计算表征表面变形和几何形状的参数。使用牙科聚合物制作的代表被检查表面的一系列模具，来获得环氧树脂铸件或指甲油复制品，这些复制品可供显微镜检查，而结构本身保持完整。在扫描电子显微镜下从环氧树脂铸件获得的图像可进一步用于三维重建和局部几何形状的计算。连续复制法是一种通用方法，可应用于对一系列结构（如分生组织、花、叶、干膜质苞片或毛状体）的复杂形状进行成像。可以研究在各种条件下生长的不同植物物种。
