Hamant Olivier, Das Pradeep, Burian Agata
Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, UCB Lyon 1, ENS de Lyon, INRA, CNRS, Lyon, France.
Department of Biophysics and Morphogenesis of Plants, University of Silesia in Katowice, Katowice, Poland.
Methods Mol Biol. 2019;1992:257-268. doi: 10.1007/978-1-4939-9469-4_17.
Analysis of meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed for an unprecedented accumulation of data on the biology of meristematic cells, as well as a better understanding of the molecular and biophysical mechanisms behind shape changes in this tissue. Here we describe in detail how to prepare shoot apices of both Arabidopsis and tomato, in order to image them over time using a confocal microscope equipped with a long distance water-dipping lens.
在过去几十年里，许多物种都对分生组织的形态和基因表达模式进行了分析。最近的实时成像技术使得关于分生组织细胞生物学的数据得到了前所未有的积累，同时也让人们对该组织形态变化背后的分子和生物物理机制有了更好的理解。在这里，我们详细描述如何制备拟南芥和番茄的茎尖，以便使用配备长距离水浸透镜的共聚焦显微镜对其进行长时间成像。
