Barmettler Gery, Ziegler Urs
Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.
Methods Mol Biol. 2019;1993:205-215. doi: 10.1007/978-1-4939-9473-1_16.
This chapter is intended to be a guide to skin studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for transmission electron microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.