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Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy.

作者信息

Barmettler Gery, Ziegler Urs

机构信息

Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.

出版信息

Methods Mol Biol. 2019;1993:205-215. doi: 10.1007/978-1-4939-9473-1_16.

DOI:10.1007/978-1-4939-9473-1_16
PMID:31148089
Abstract

This chapter is intended to be a guide to skin studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for transmission electron microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.

摘要

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