Department of Materials Science and Engineering, Jinan University, Guangzhou, China.
The first affiliated hospital of Jinan University, Jinan University, Guangzhou, China.
J Biomed Mater Res A. 2019 Oct;107(10):2272-2281. doi: 10.1002/jbm.a.36735. Epub 2019 Jun 17.
Bioadaptive nanostructure coatings of hydroxyapatite (HAP) and TiO on titanium (Ti) implants are essential for biomaterial-tissue osteointegration. However, there is no specific report, so far, that focuses on the different influences of the two bioadaptive coatings on preosteoblast behaviors. Herein, adhesion, proliferation, and osteogenic potential of preosteoblast on HAP and TiO coatings with nanorod array topography were studied. XRD, TEM, and SAED analysis indicated that rod-like HAP nanoarray and anatase TiO nanoarray coatings were fabricated successfully, and there was insignificant difference in roughness and fibronectin adsorption of the two coatings. Adhesion and proliferation of MC3T3-E1 cells on the two coatings were of no significant difference, besides a larger projected area of the cells on HAP coating. MC3T3-E1 cells cultured on the HAP coating displayed significantly higher expression of runt-related transcription factor-2 (Runx2), osteocalcin (OCN) and collagen type-1 (Col I) after culture for 21 days compared with those on TiO coating, except alkaline phosphatase (ALP). This study provides beneficial suggestion for intelligent selection of biocoatings.
生物适应性纳米结构涂层的羟基磷灰石（HAP）和 TiO 在钛（Ti）植入物是必不可少的生物材料组织骨整合。然而，到目前为止，还没有专门的报告，重点介绍了两种生物适应性涂层对成骨前体细胞行为的不同影响。在此，研究了成骨前体细胞在具有纳米棒阵列形貌的 HAP 和 TiO 涂层上的黏附、增殖和成骨潜能。XRD、TEM 和 SAED 分析表明，成功制备了棒状 HAP 纳米阵列和锐钛矿 TiO 纳米阵列涂层，两种涂层的粗糙度和纤连蛋白吸附没有明显差异。MC3T3-E1 细胞在两种涂层上的黏附和增殖没有显著差异，除了 HAP 涂层上细胞的投影面积更大。MC3T3-E1 细胞在 HAP 涂层上培养 21 天后，与在 TiO 涂层上相比，Runt 相关转录因子 2（Runx2）、骨钙素（OCN）和胶原 I（Col I）的表达显著升高，碱性磷酸酶（ALP）除外。这项研究为智能选择生物涂层提供了有益的建议。
