Department of Materials Science and Engineering, Jinan University, Guangzhou, 510632, China.
Department of Materials Science and Engineering, Jinan University, Guangzhou, 510632, China.
Colloids Surf B Biointerfaces. 2018 Nov 1;171:40-48. doi: 10.1016/j.colsurfb.2018.06.046. Epub 2018 Jun 21.
How the surface topography of hydroxyapatite (HAP) coatings remodels hard tissue is of utmost importance and a contributing factor to ambiguity regarding this subject. Here, HAP coatings with different topographies of a rod-like nanoarray with c-axis orientation and a flake-like micro-flower array with a(b)-axis orientation on a Ti substrate were synthesized via hydrothermal-electrodeposition by controlling the concentration of electrolytes. XRD, TEM and SAED analyses indicated that the rod-like HAP nanoarray was predominant with an orientation of (001), while the HAP micro-flower samples were based on an orientation of (100). Compared to the flake-like HAP, the rod-like nanoarray HAP possessed better hydrophilic properties and lower roughness, which not only enhanced adsorption of specific fibronectin proteins but also promoted the spreading and growth of MC3T3-E1 cells. Runx2, alkaline phosphatase, collagen and osteocalcin were also analyzed by RT-PCR on the two distinctive HAP-coated samples. MC3T3-E1 cells on the rod-like nanoarray coating had higher osteo-related gene expression. This finding suggested that the ordered assembly structure of the HAP might cause topography-dependent coordination with biomolecules for enhancing osteoblast-like cell proliferation and osteogenic differentiation. This study provided an understanding of the surface's features for biomaterials to ensure better bioactivity.
羟基磷灰石（HAP）涂层的表面形貌如何重塑硬组织是至关重要的，也是导致这一主题模糊不清的一个因素。在这里，通过控制电解质浓度，通过水热-电沉积在 Ti 基底上合成了具有 c 轴取向的棒状纳米阵列和具有（a）-（b）轴取向的片状微花阵列的不同形貌的 HAP 涂层。XRD、TEM 和 SAED 分析表明，棒状 HAP 纳米阵列以（001）取向为主，而 HAP 微花样品则基于（100）取向。与片状 HAP 相比，棒状纳米阵列 HAP 具有更好的亲水性和更低的粗糙度，这不仅增强了特定纤维连接蛋白的吸附，还促进了 MC3T3-E1 细胞的铺展和生长。通过 RT-PCR 对两种不同形貌的 HAP 涂层样品进行了 Runx2、碱性磷酸酶、胶原和骨钙素分析。在棒状纳米阵列涂层上的 MC3T3-E1 细胞具有更高的与骨相关的基因表达。这一发现表明，HAP 的有序组装结构可能会与生物分子发生形貌依赖的协调作用，从而增强成骨样细胞的增殖和成骨分化。本研究为生物材料的表面特征提供了深入的了解，以确保更好的生物活性。
