Suganthi Soundrapandian, Sivaraj Ramasamy, Enoch Israel V M V
Department of Chemistry, Karunya Institute of Technology and Sciences (Deemed-to-be University) , Coimbatore , Tamil Nadu , India.
Nanotoxicology Research Lab, Department of Nanoscience, Karunya Institute of Technology and Sciences (Deemed-to-be University) , Coimbatore , Tamil Nadu , India.
Nucleosides Nucleotides Nucleic Acids. 2019;38(11):858-873. doi: 10.1080/15257770.2019.1618469. Epub 2019 May 31.
The capacity to control quadruplex formation, especially in cancer cells, is captivating and entails a reasonable comprehension of the ligand-G-quadruplex binding. Herein, we report an iminopyrenyl-β-cyclodextrin conjugate interacting with duplex and G-quadrulex DNAs. In addition, the host: guest association of the established G-quadruplex binder, berberine, with the β-cyclodextrin derivative is studied employing 2-D ROESY. NMR, UV-visible, and fluorescence spectroscopic techniques are utilized to explore the β-cyclodextrin conjugate's interaction with the quadruplexes. The Binding constants are accounted for the association of the ligands to each of the DNAs viz., calf thymus DNA (duplex), , , and (quadruplexes). The modulation of the iminopyrenyl-β-cyclodextrin binding to the DNAs are observed when berberine is loaded in the host molecule. A vivid distinction between the interactions of the ligands with duplex and quadruplex structures is inferred. Berberine-loaded iminopyrenyl-β-cyclodextrin shows a higher affinity for binding to .
控制四链体形成的能力，尤其是在癌细胞中的这种能力，极具吸引力，并且需要对配体与G-四链体的结合有合理的理解。在此，我们报道了一种亚氨基芘基-β-环糊精共轭物与双链和G-四链体DNA的相互作用。此外，利用二维旋转核Overhauser效应光谱（2-D ROESY）研究了已确定的G-四链体结合剂黄连素与β-环糊精衍生物的主客体缔合。利用核磁共振（NMR）、紫外可见光谱和荧光光谱技术来探究β-环糊精共轭物与四链体的相互作用。结合常数用于说明配体与每种DNA即小牛胸腺DNA（双链）、 、 和 （四链体）的缔合情况。当黄连素负载在主体分子中时，观察到亚氨基芘基-β-环糊精与DNA结合的调制情况。推断出配体与双链和四链体结构相互作用之间存在明显差异。负载黄连素的亚氨基芘基-β-环糊精对 显示出更高的结合亲和力。
