绿色合成金纳米颗粒在医学消毒应用中的消毒效果
Disinfection efficacy of green synthesized gold nanoparticles for medical disinfection applications.
作者信息
Huang Qionghui, Luo Aihong, Jiang Lijuan, Zhou Yan, Yang Yanting, Liu Qiong, Zhang Chunfang
机构信息
Central sterile supply department, Xiangya Hospital, Central South University 410008.
Department of thoracic surgery, Xiangya Hospital, Central South University 410008.
出版信息
Afr Health Sci. 2019 Mar;19(1):1441-1448. doi: 10.4314/ahs.v19i1.17.
BACKGROUND
In recent times, biosyntheses of metal nanoparticles were used for several life rescue applications. In this study, Dillenia indica leaf aqueous extract was utilized for the synthesis of gold nanoparticles.
OBJECTIVE
To test anti-microbial properties of biologically fabricated gold nanoparticles.
METHODS
Gold nanoparticles were efficiently prepared by making use of aqueous leaf extract of Dillenia indica. The excitation of formed AuNPs was confirmed using UV-Vis spectrophotometer. In particular, absorption spectra of AuNPs exhibited a well-defined SPR band centered at around 530 nm.
RESULTS
The high-resolution Scanning Electron Microscope (SEM) results of the obtained AuNPs confirmed the formation of particles with a size range of 5-50 nm. The ultra-high resolution TEM (UHRTEM) images displayed clear lattice fringes on the particle surfaces. Single crystalline nature of the biosynthesized AuNPs was represented by means of selected-area electron diffraction pattern.
CONCLUSION
The antibacterial activity of AuNPs revealed significant activity towards both gram negative and gram positive bacteria signifying their potential disinfection related applications in medicine and biology.
背景
近年来，金属纳米粒子的生物合成被用于多种生命救援应用。在本研究中，五桠果叶水提取物被用于合成金纳米粒子。
目的
测试生物制备的金纳米粒子的抗菌性能。
方法
利用五桠果叶水提取物高效制备金纳米粒子。使用紫外可见分光光度计确认所形成的金纳米粒子的激发。特别是，金纳米粒子的吸收光谱在530nm左右呈现出明确的表面等离子体共振（SPR）带。
结果
所获得的金纳米粒子的高分辨率扫描电子显微镜（SEM）结果证实形成了尺寸范围为5-50nm的颗粒。超高分辨率透射电子显微镜（UHRTEM）图像显示颗粒表面有清晰的晶格条纹。通过选区电子衍射图案表征了生物合成的金纳米粒子的单晶性质。
结论
金纳米粒子的抗菌活性对革兰氏阴性菌和革兰氏阳性菌均显示出显著活性，表明其在医学和生物学中潜在的消毒相关应用。
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