Department of Chemistry, Jagannath University, Dhaka 1100, Bangladesh.
Center for Nanotechnology, Department of Natural Sciences, Coppin State University, 2500 W. North Ave, Baltimore, MD 21216, USA.
Molecules. 2020 Oct 18;25(20):4773. doi: 10.3390/molecules25204773.
In this report, we discussed rapid, facile one-pot green synthesis of gold and silver nanoparticles (AuNPs and AgNPs) by using tuber extract of , and evaluated their antibacterial activity. AuNPs and AgNPs were synthesized by mixing their respective precursors (AgNO and HAuCl) with tuber extract of as the bio-reducing agent. Characterization of AuNPs and AgNPs were confirmed by applying UV-vis spectroscopy, field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDS). From UV-vis characterization, surface plasmon resonance spectra were found at 530 nm for AuNPs and 446 nm for AgNPs. XRD data confirmed that both synthesized nanoparticles were face-centered cubic in crystalline nature, and the average crystallite sizes for the assign peaks were 13.3 nm for AuNPs and 22.48 nm for AgNPs. FTIR data evaluated the characteristic peaks of different phytochemical components of tuber extract, which acted as the reducing agent, and possibly as stabilizing agents. The antibacterial activity of synthesized AuNPs and AgNPs were examined in Muller Hinton agar, against two Gram-positive and four Gram-negative bacteria through the disc diffusion method. AuNPs did not show any inhibitory effect, while AgNPs showed good inhibitory effect against both Gram-positive and Gram-negative bacteria.
在本报告中，我们讨论了通过使用块茎提取物快速、简便地一锅绿色合成金和银纳米粒子（AuNPs 和 AgNPs），并评估了它们的抗菌活性。AuNPs 和 AgNPs 的合成是通过将各自的前体（AgNO 和 HAuCl）与块茎提取物混合作为生物还原剂来实现的。AuNPs 和 AgNPs 的特征通过应用紫外可见光谱、场发射扫描电子显微镜（FESEM）、X 射线衍射（XRD）分析、傅里叶变换红外光谱（FTIR）和能谱分析（EDS）来确认。从紫外可见特征来看，AuNPs 的表面等离子体共振光谱在 530nm 处，AgNPs 的在 446nm 处。XRD 数据证实了两种合成的纳米粒子均具有面心立方的晶体性质，且分配峰的平均晶粒尺寸分别为 13.3nm 的 AuNPs 和 22.48nm 的 AgNPs。FTIR 数据评估了块茎提取物中不同植物化学成分的特征峰，这些成分起到了还原剂的作用，并可能起到了稳定剂的作用。通过圆盘扩散法，在 Muller Hinton 琼脂中，对两种革兰氏阳性菌和四种革兰氏阴性菌检测了合成的 AuNPs 和 AgNPs 的抗菌活性。AuNPs 没有显示出任何抑制作用，而 AgNPs 对革兰氏阳性菌和革兰氏阴性菌均显示出良好的抑制作用。
