Jeong Soon Woo, Park Yoo Min, Jo Sung Hee, Lee Seok Jae, Kim Yong Tae, Lee Kyoung G
Nano-Bio Application Team, National Nanofab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, South Korea.
Department of Chemical Engineering & Biotechnology, Korea Polytechnic University, 237 Sangidaehak-ro, Siheung-si, Gyeonggi-do 15073, South Korea.
Biomicrofluidics. 2019 May 22;13(3):034111. doi: 10.1063/1.5093752. eCollection 2019 May.
The on-site recovery of nucleic acid from foodborne bacteria is in high demand to further understand on-site molecular diagnosis, which is especially applicable in developing countries. Here, we first proposed a smartphone operable centrifugal system (SOCS) for nucleic acid extraction with the assistance of a low power consumable motor and hydrogel beads. The SOCS consists of a centrifugal motor, 3D-printed cartridge, a nucleic acid collection column, and a smartphone. The SOCS shows excellent DNA extraction performance within 6 min, and it can operate more than 100 times using a smartphone. The purified effluent DNA was accumulated in the nucleic acid collection column. The performance of the SOCS was confirmed by amplifying the recovered DNA from O157:H7. Moreover, the artificially inoculated food and blood samples also confirmed the performance of SOCS. The proposed SOCS provides an on-site operable nucleic acid separation platform in terms of simplicity, easy usability, cost-effectiveness, and portability in pathogenic point-of-care diagnostics.
为了进一步了解现场分子诊断，对食源细菌进行核酸的现场回收需求很高，这在发展中国家尤为适用。在此，我们首次提出了一种可通过智能手机操作的离心系统（SOCS），借助低功耗的一次性电机和水凝胶珠进行核酸提取。SOCS由一个离心电机、3D打印盒、一个核酸收集柱和一部智能手机组成。SOCS在6分钟内展现出优异的DNA提取性能，并且使用智能手机可操作100多次。纯化后的流出DNA积聚在核酸收集柱中。通过扩增从O157:H7回收的DNA，证实了SOCS的性能。此外，人工接种的食品和血液样本也证实了SOCS的性能。所提出的SOCS在致病性即时诊断中，在简单性、易用性、成本效益和便携性方面提供了一个现场可操作的核酸分离平台。
