Dorozhkova I R, Karachunskiĭ M A, Kiazimova L G, Bibergal' E A
Zh Mikrobiol Epidemiol Immunobiol. 1987 Jun(6):25-9.
Wide-range microbiological study (bacterioscopy, inoculation, biological assays) of 114 lung tuberculomas excised from 107 patients has revealed a pronounced variability and sharply decreased viability of mycobacterial populations vegetating in caseous foci. Differences in the frequency and character of the detection of Mycobacterium tuberculosis and their altered forms, arising from the use of three above-mentioned methods of microbiological investigation, were noted. Bacterioscopy proved to be more informative with respect to the detection of the bacterial forms of M. tuberculosis. Biological assay was highly sensitive with respect to the L-forms of M. tuberculosis and permitted the detection of the persisting forms of this infective agent, contained in caseous foci and not detected by the method of inoculations. To evaluate the actual state of the mycobacterial population in the focus of tuberculous lesion, the use of a complex of microbiological methods for the detection of typical and biologically altered forms of M. tuberculosis is necessary.
对从107例患者身上切除的114个肺结核瘤进行的广泛微生物学研究（细菌学检查、接种、生物学测定）显示，在干酪样病灶中生长的分枝杆菌群体具有明显的变异性，其活力急剧下降。注意到使用上述三种微生物学研究方法在检测结核分枝杆菌及其变异形式的频率和特征方面存在差异。细菌学检查在检测结核分枝杆菌的细菌形式方面更具信息性。生物学测定对结核分枝杆菌的L型高度敏感，并能检测出干酪样病灶中所含的这种感染因子的持续形式，而接种法无法检测到这些形式。为了评估结核病变病灶中分枝杆菌群体的实际状态，有必要使用一套微生物学方法来检测结核分枝杆菌的典型形式和生物学变异形式。
